ANGPTL7 Human

ANGPTL7 is composed of an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain . This structural composition is crucial for its function. The protein is involved in various biological processes, including the regulation of angiogenesis, which is the formation of new blood vessels from pre-existing ones .

ANGPTL7 is expressed in several tissues, including the corneal stroma, trabecular meshwork, and sclera . Its expression is notably elevated in the aqueous humor of individuals with glaucoma . This suggests a potential role in the pathophysiology of this eye condition.

The biological activity of ANGPTL7 has been studied in various experimental settings. For instance, it has been shown to promote the expansion of E16 rat liver mononuclear cells in vitro . This activity is measured in the presence of other recombinant proteins such as mouse SCF/c-kit ligand, mouse thrombopoietin, and mouse Flt-3 ligand .

Recombinant human ANGPTL7 is typically produced using Chinese Hamster Ovary (CHO) cell lines . The recombinant protein is purified to a high degree, with a purity greater than 95% as determined by SDS-PAGE under reducing conditions . The endotoxin level is kept below 1.0 EU per microgram of protein, ensuring its suitability for various experimental applications .

Recombinant ANGPTL7 is used in various research applications, including studies on angiogenesis, glaucoma, and other conditions where its regulatory role in blood vessel formation and maintenance is of interest . The protein is available in different formulations, with or without carrier proteins like Bovine Serum Albumin (BSA), depending on the specific requirements of the experimental setup .

The recombinant protein is typically lyophilized and can be reconstituted in PBS . It is shipped at ambient temperature and should be stored at -20 to -70°C to maintain its stability. Once reconstituted, it can be stored at 2 to 8°C for up to one month under sterile conditions .