ANGPTL2 Human
Sterile filtered colorless solution.
Greater than 85.0% as determined by SDS-PAGE.
Description
ANGPTL2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 493 amino acids ( 22-493 a.a.) including a 20 a.a N-terminal His tag. The total molecular mass is 57.1kDa (calculated).
Product Specs
Recombinant Human ANGPTL2, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 493 amino acids (22-493 a.a.). This includes a 20 a.a. N-terminal His tag, resulting in a total molecular mass of 57.1kDa (calculated).
The product appears as a clear, colorless solution that has been sterilized by filtration.
The ANGPTL2 protein solution is provided at a concentration of 0.5mg/ml and is buffered in a solution of 20mM Tris HCL (pH 7-8) with 10% glycerol.
For short-term storage (2-4 weeks), the product should be kept refrigerated at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To further enhance stability during long-term storage, adding a carrier protein (0.1% HSA or BSA) is advisable. It is important to minimize repeated freeze-thaw cycles to preserve product integrity.
The purity of the ANGPTL2 protein is determined to be greater than 85.0% based on SDS-PAGE analysis.
MGSSHHHHHH SSGLVPRGSH MGQEDGFEGT EEGSPREFIY LNRYKRAGES QDKCTYTFIV PQQRVTGAIC VNSKEPEVLL ENRVHKQELE LLNNELLKQK RQIETLQQLV EVDGGIVSEV KLLRKESRNM NSRVTQLYMQ LLHEIIRKRD NALELSQLEN RILNQTADML QLASKYKDLE HKYQHLATLA HNQSEIIAQL EEHCQRVPSA RPVPQPPPAA PPRVYQPPTY NRIINQISTN EIQSDQNLKV LPPPLPTMPT LTSLPSSTDK PSGPWRDCLQ ALEDGHDTSS IYLVKPENTN RLMQVWCDQR HDPGGWTVIQ RRLDGSVNFF RNWETYKQGF GNIDGEYWLG LENIYWLTNQ GNYKLLVTME DWSGRKVFAE YASFRLEPES EYYKLRLGRY HGNAGDSFTW HNGKQFTTLD RDHDVYTGNC AHYQKGGWWY NACAHSNLNG VWYRGGHYRS RYQDGVYWAE FRGGSYSLKK VVMMIRPNPN TFH
Product Science Overview
ANGPTL2 is a secreted glycoprotein that does not bind to the Tie-1 or Tie-2 receptors, which are typically associated with angiopoietins . The mature human ANGPTL2 shares 89% sequence identity with both mouse and rat homologs . It is widely expressed in many tissues and its expression is induced by chronic, but not acute, hypoxia .
ANGPTL2 has been shown to induce sprouting in endothelial cells, although it is not mitogenic . It plays a significant role in promoting the expansion of E16 rat liver mononuclear cells in vitro, especially in the presence of other growth factors such as Recombinant Mouse SCF/c-kit Ligand, Recombinant Mouse Thrombopoietin/Tpo, and Recombinant Mouse Flt-3 Ligand . The protein is also involved in various inflammatory processes and has been linked to metabolic regulation.
Recombinant human ANGPTL2 is typically produced using a mouse myeloma cell line, NS0-derived human Angiopoietin-like Protein 2/ANGPTL2 protein . The protein is purified to a high degree, with a purity greater than 75% as determined by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining . The endotoxin level is kept below 0.10 EU per 1 μg of the protein by the LAL method .
The recombinant protein is typically lyophilized from a 0.2 μm filtered solution and can be reconstituted at 200 μg/mL in water . It is shipped at ambient temperature and should be stored at -20 to -70 °C to maintain its stability. Once reconstituted, it can be stored under sterile conditions at 2 to 8 °C for up to one month or at -20 to -70 °C for up to three months .
