ANGPT2 Human
Chinese Hamster Ovary Cells (CHO).
Angiopoietin-2, Angiopoietin2, ANGPT2, ANG2, ANG-2, ANGPT-2.
Sterile filtered colorless solution.
Greater than 90.0% as determined by SDS-PAGE.
Description
ANGPT2 produced in Chinese hamster ovary (CHO) cells by recombinant DNA technology is a single, polypeptide chain (19-496 a.a.) and fused to a 6 aa His Tag at C-terminus containing a total of 484 amino acids and having a molecular mass of 55.7kDa.
ANGPT2 shows multiple bands between 50-100kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.
Product Specs
Angiopoietin-2, also known as ANGPT2, competes with angiopoietin-1 (ANGPT1) for binding to the TIE2 receptor. This competition blocks ANGPT1-induced TIE2 autophosphorylation, a process essential for the formation of new blood vessels (vasculogenesis). Therefore, ANGPT2 acts as a natural antagonist to ANGPT1. In the absence of ANGPT2, ANGPT2 can induce tyrosine phosphorylation of TEK/TIE2. Interestingly, in environments lacking VEGF, a crucial angiogenic inducer, ANGPT2 triggers programmed cell death (apoptosis) in endothelial cells, leading to the regression of blood vessels. However, when both ANGPT2 and VEGF are present, they work together to promote the movement (migration) and multiplication (proliferation) of endothelial cells, thereby creating conditions that favor angiogenesis.
Recombinant human ANGPT2 is produced in Chinese hamster ovary (CHO) cells using recombinant DNA technology. It is a single polypeptide chain encompassing amino acids 19 to 496 of the ANGPT2 protein sequence. A 6-amino acid histidine tag (His-tag) is fused to the C-terminus for purification purposes. The resulting protein consists of 484 amino acids and has a molecular weight of 55.7 kilodaltons (kDa). When analyzed by SDS-PAGE under reducing conditions, ANGPT2 appears as multiple bands ranging from 50 to 100 kDa. The protein is purified using proprietary chromatographic techniques.
The product is a sterile-filtered solution that appears colorless.
The ANGPT2 protein solution is provided at a concentration of 0.25 milligrams per milliliter (mg/ml) in a buffer consisting of phosphate-buffered saline (PBS) at pH 7.4 and 10% glycerol.
For short-term storage (up to 2-4 weeks), the product can be stored at 4 degrees Celsius. For long-term storage, it is recommended to freeze the protein at -20 degrees Celsius. Adding a carrier protein such as albumin (HSA or BSA) to a final concentration of 0.1% is advisable for extended storage periods. Repeated freezing and thawing of the protein should be avoided.
The purity of the ANGPT2 protein is greater than 90% as determined by SDS-PAGE analysis.
Angiopoietin-2, Angiopoietin2, ANGPT2, ANG2, ANG-2, ANGPT-2.
Chinese Hamster Ovary Cells (CHO).
YNNFRKSMDS IGKKQYQVQH GSCSYTFLLP EMDNCRSSSS PYVSNAVQRD APLEYDDSVQ
RLQVLENIME NNTQWLMKLE NYIQDNMKKEMVEIQQNAVQ NQTAVMIEIG TNLLNQTAEQ
TRKLTDVEAQ VLNQTTRLEL QLLEHSLSTN KLEKQILDQT SEINKLQDKN
SFLEKKVLAMEDKHIIQLQS IKEEKDQLQV LVSKQNSIIE ELEKKIVTAT VNNSVLQKQQ
HDLMETVNNL LTMMSTSNSA KDPTVAKEEQ ISFRDCAEVFKSGHTTNGIY TLTFPNSTEE
IKAYCDMEAG GGGWTIIQRR EDGSVDFQRT WKEYKVGFGN PSGEYWLGNE FVSQLTNQQR
YVLKIHLKDWEGNEAYSLYE HFYLSSEELN YRIHLKGLTG TAGKISSISQ PGNDFSTKDG
DNDKCICKCS QMLTGGWWFD ACGPSNLNGM YYPQRQNTNKFNGIKWYYWK GSGYSLKATT MMIRPADFHH HHHH
Product Science Overview
Angiopoietin-2 is composed of 478 amino acids and has a molecular weight of approximately 56 kDa. It is produced as a precursor protein that undergoes post-translational modifications, including glycosylation, to become fully functional . The protein is predominantly expressed in endothelial cells, which line the interior surface of blood vessels .
Angiopoietin-2 functions by binding to the Tie-2 receptor, a tyrosine kinase receptor found on the surface of endothelial cells. This interaction can either promote or inhibit angiogenesis, depending on the presence of other factors. In the absence of Angiopoietin-1 (Ang-1), Ang-2 acts as an antagonist, inhibiting Tie-2 signaling and leading to blood vessel destabilization and increased vascular permeability . However, in the presence of Ang-1, Ang-2 can act as an agonist, promoting angiogenesis and blood vessel maturation .
Angiopoietin-2 is upregulated in various pathological conditions, including cancer, inflammation, and cardiovascular diseases. Its ability to modulate blood vessel formation and permeability makes it a key player in tumor angiogenesis, where it facilitates the growth of new blood vessels to supply nutrients to the tumor . Additionally, Ang-2 is implicated in inflammatory diseases, where it contributes to the regulation of immune cell infiltration and tissue edema .
Given its significant role in angiogenesis and inflammation, Angiopoietin-2 is a target for therapeutic interventions. Recombinant human Angiopoietin-2 (rhAng-2) is used in research to study its biological functions and potential therapeutic applications. It is produced using recombinant DNA technology, where the ANGPT2 gene is inserted into a host cell line, such as CHO (Chinese Hamster Ovary) cells, to produce the protein in large quantities .
