ALDH2 Mouse, Active
Greater than 95% as determined by SDS-PAGE.
Description
ALDH2 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 523 amino acids (20-519) and having a molecular mass of 56.8kDa.
ALDH2 Mouse is fused to a 23 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
This recombinant ALDH2 protein was expressed in E. coli and purified. It is a single, non-glycosylated polypeptide chain with a molecular weight of 56.8 kDa. The protein sequence includes amino acids 20-519 of the mouse ALDH2 protein, along with a 23 amino acid His-tag at the N-terminus.
Purification was achieved using proprietary chromatographic techniques.
MGSSHHHHHH SSGLVPRGSH MGSSAAATSA VPAPNHQPEV FCNQIFINNE WHDAVSRKTF PTVNPSTGEV ICQVAEGNKE DVDKAVKAARAAFQLGSPWR RMDASDRGRL LYRLADLIER DRTYLAALET LDNGKPYVIS YLVDLDMVLK CLRYYAGWAD KYHGKTIPID GDFFSYTRHEPVGVCGQIIP WNFPLLMQAW KLGPALATGN VVVMKVAEQT PLTALYVANL IKEAGFPPGV VNIVPGFGPT AGAAIASHEG VDKVAFTGSTEVGHLIQVAA GSSNLKRVTL ELGGKSPNII MSDADMDWAV EQAHFALFFN QGQCCCAGSR TFVQENVYDE FVERSVARAK SRVVGNPFDSRTEQGPQVDE TQFKKILGYI KSGQQEGAKL LCGGGAAADR GYFIQPTVFG DVKDGMTIAK EEIFGPVMQI LKFKTIEEVV GRANDSKYGLAAAVFTKDLD KANYLSQALQ AGTVWINCYD VFGAQSPFGG YKMSGSGREL GEYGLQAYTE VKTVTVKVPQ KNS.
Product Science Overview
Aldehyde Dehydrogenase 2 (ALDH2) is a crucial enzyme in the aldehyde dehydrogenase family, playing a significant role in the oxidative pathway of alcohol metabolism. This enzyme is responsible for converting acetaldehyde, a toxic byproduct of alcohol metabolism, into acetic acid, which is less harmful to the body .
The mouse recombinant ALDH2 is produced in E. coli and is a single, non-glycosylated polypeptide chain containing 523 amino acids. It has a molecular mass of approximately 56.8 kDa and includes a 23 amino acid His-tag at the N-terminus for purification purposes . The enzyme is expressed in various tissues, including the liver, where it plays a pivotal role in detoxifying aldehydes generated from alcohol and other metabolic processes .
ALDH2 is the second enzyme in the major oxidative pathway of alcohol metabolism. It is essential for the detoxification of acetaldehyde, which, if accumulated, can cause cellular damage and contribute to conditions such as alcohol-induced liver disease . The enzyme’s activity is crucial for preventing acetaldehyde-induced cell injury and inhibiting the activation of stress signals .
Mutations in the ALDH2 gene can lead to a deficiency in enzyme activity, which is particularly prevalent in East Asian populations. This deficiency is associated with an increased risk of alcohol-related health issues, including a higher frequency of acute alcohol intoxication and a predisposition to certain cancers . The recombinant form of ALDH2 is used in research to study these effects and to develop potential therapeutic interventions.
Recombinant ALDH2 is widely used in biochemical and pharmacological research. It serves as a model to understand the enzyme’s structure-function relationship, investigate the impact of genetic mutations, and explore potential drug interactions. The recombinant enzyme is also employed in studies aimed at developing treatments for conditions related to ALDH2 deficiency .
