AKR7A3 Human, His
Description
AKR7A3 Human Recombinant fused to a 39 amino acids His Tag at N-terminal produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 370 amino acids (1-331 a.a.) and having a molecular mass of 41.6 kDa. The AKR7A3 is fused to a 39 amino acid His tag at n-terminal and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Aldo-Keto Reductase Family 7 Member A3, commonly referred to as AKR7A3, is a protein-coding gene that plays a significant role in the detoxification of aldehydes and ketones. This protein is part of the aldo-keto reductase superfamily, which consists of enzymes that catalyze the reduction of aldehydes and ketones to their corresponding alcohols using NADPH as a cofactor .
The AKR7A3 gene is located on chromosome 1 and encodes a protein that is 331 amino acids long. The recombinant form of this protein, tagged with a polyhistidine (His) tag, is expressed in Escherichia coli for research and industrial purposes. The His tag facilitates the purification of the protein using affinity chromatography .
AKR7A3 is known for its ability to reduce the dialdehyde protein-binding form of aflatoxin B1 to the non-binding aflatoxin B1 dialcohol. This activity is crucial in protecting the liver from the toxic and carcinogenic effects of aflatoxin B1, a potent hepatocarcinogen . The enzyme’s activity is measured by its ability to catalyze the oxidation of NADPH, and its specific activity is expressed in units per milligram of protein .
The detoxification role of AKR7A3 is vital in various metabolic pathways, including the biotransformation of xenobiotics and the metabolism of endogenous compounds. The enzyme’s ability to neutralize harmful aldehydes and ketones helps maintain cellular homeostasis and protect against oxidative stress .
Mutations or dysregulation of the AKR7A3 gene have been associated with certain diseases, including congenital symmetric circumferential skin creases and multiple benign circumferential skin creases on limbs . Understanding the function and regulation of AKR7A3 can provide insights into the development of therapeutic strategies for these conditions.
