AKR1B10 Human

Aldo-Keto Reductase Family 1 Member B10 Human Recombinant
Cat. No.
BT15984
Source
Escherichia Coli.
Synonyms
HIS, HSI, ARL1, ARL-1, ALDRLn, AKR1B11, AKR1B12, MGC14103, AKR1B10, Aldo-keto reductase family 1 member B10, Aldose reductase-like, Aldose reductase-related protein, ARP, hARP, Small intestine reductase, SI reductase.
Appearance
Sterile Filtered clear colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

AKR1B10 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 316 amino acids (1-316 a.a) and having a molecular mass of 36 kDa.
The AKR1B10 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
AKR1B10 is an enzyme that exhibits a strong ability to reduce both aliphatic and aromatic aldehydes, while demonstrating less activity towards hexoses. Notably expressed in the adrenal gland, small intestine, and colon, AKR1B10 is believed to play a significant role in the development of liver cancer. Functioning as a monomeric protein, it effectively catalyzes the reduction of aromatic and aliphatic aldehydes and ketones. AKR1B10 is found extensively in various human tissues, particularly in the small intestine, colon, and adrenal gland. Implicated in the pathogenesis of diabetic complications, AKR1B10 also displays elevated expression levels in several types of human tumors, including liver, breast, and lung cancers, pointing to its involvement in cancer development and progression.
Description
Recombinant human AKR1B10, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 316 amino acids (amino acids 1-316). It has a molecular weight of 36 kDa. The purification process of AKR1B10 involves the use of proprietary chromatographic methods.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The AKR1B10 solution is prepared with 20mM Tris-HCl at a pH of 8 and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product should be kept at 4°C. For extended storage, freezing at -20°C is recommended. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of the product is determined to be greater than 95.0% based on SDS-PAGE analysis.
Synonyms
HIS, HSI, ARL1, ARL-1, ALDRLn, AKR1B11, AKR1B12, MGC14103, AKR1B10, Aldo-keto reductase family 1 member B10, Aldose reductase-like, Aldose reductase-related protein, ARP, hARP, Small intestine reductase, SI reductase.
Source
Escherichia Coli.
Amino Acid Sequence
MATFVELSTK AKMPIVGLGT WKSPLGKVKE AVKVAIDAGY RHIDCAYVYQ NEHEVGEAIQ EKIQEKAVKR EDLFIVSKLW PTFFERPLVRKAFEKTLKDL KLSYLDVYLI HWPQGFKSGD DLFPKDDKGN AIGGKATFLD AWEAMEELVD EGLVKALGVS NFSHFQIEKL LNKPGLKYKP VTNQVECHPY LTQEKLIQYC HSKGITVTAY SPLGSPDRPW AKPEDPSLLE DPKIKEIAAK HKKTAAQVLI RFHIQRNVIV IPKSVTPARIVENIQVFDFK LSDEEMATIL SFNRNWRACN VLQSSHLEDY PFDAEY.

Product Science Overview

Gene and Protein Structure

The AKR1B10 gene is located on chromosome 7q33 in humans . The gene encodes a cytosolic enzyme consisting of 316 amino acids . The enzyme is NADPH-dependent and efficiently reduces a variety of aliphatic and aromatic aldehydes, as well as some drug ketones .

Expression and Function

AKR1B10 is predominantly expressed in the gastrointestinal tract, including the small intestine and colon . It is also found in the adrenal gland and liver, albeit at lower levels . The enzyme plays a crucial role in detoxifying cytotoxic carbonyls, modulating retinoic acid levels, and regulating cellular fatty acid synthesis and lipid metabolism .

Role in Cancer

AKR1B10 is overexpressed in several types of solid tumors, such as hepatocellular carcinoma (HCC), lung cancer, and breast cancer . Its overexpression is associated with the formation and development of carcinomas through multiple mechanisms, including detoxification of cytotoxic carbonyls and modulation of retinoic acid levels . Studies have suggested that AKR1B10 could serve as a useful biomarker for cancer diagnosis and a potential target for cancer treatment .

Recombinant AKR1B10

Recombinant human AKR1B10 is produced using recombinant DNA technology, which involves inserting the human AKR1B10 gene into a suitable expression system, such as bacteria or yeast, to produce the enzyme in large quantities. This recombinant enzyme is used in various research applications, including studies on its role in cancer and its potential as a therapeutic target .

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