AKR7A3, Human
Escherichia Coli.
AFAR2, Aflatoxin B1 aldehyde reductase member 3, AFB1 aldehyde reductase 2, AFB1-AR 2, AKR7A3.
Greater than 90.0% as determined by SDS-PAGE.
Description
AKR7A3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 331 amino acids (1-331) and having a molecular mass of 37.7 kDa.
AKR7A3 is purified by proprietary chromatographic techniques.
Product Specs
Aldo-Keto Reductase Family 7 Member A3, also known as AKR7A3, is an enzyme involved in the detoxification of aldehydes and ketones. AKR7A3 converts the dialdehyde form of aflatoxin B1 (AFB1), which binds to proteins, into the non-binding AFB1 dialcohol. This process helps protect the liver from the toxic and carcinogenic effects of AFB1, a potent liver carcinogen.
Recombinant human AKR7A3 produced in E. coli is a single, non-glycosylated polypeptide chain consisting of 331 amino acids (1-331) with a molecular weight of 37.7 kDa. The protein is purified using proprietary chromatographic techniques.
AKR7A3 is supplied as a 1 mg/ml solution in 20mM Tris-HCl buffer (pH 8.5) containing 10% glycerol.
Purity is greater than 90.0% as determined by SDS-PAGE analysis.
Specific activity is greater than 800 pmol/min/µg, defined as the amount of enzyme that catalyzes the reduction of 1.0 pmole of 1,2-Naphthoquinone per minute in the presence of NADPH at pH 7.0 and 25°C.
AFAR2, Aflatoxin B1 aldehyde reductase member 3, AFB1 aldehyde reductase 2, AFB1-AR 2, AKR7A3.
Escherichia Coli.
MSRQLSRARP ATVLGAMEMG RRMDAPTSAA VTRAFLERGH TEIDTAFVYS EGQSETILGG LGLRLGGSDC RVKIDTKAIP LFGNSLKPDS LRFQLETSLK RLQCPRVDLF YLHMPDHSTP VEETLRACHQ LHQEGKFVEL GLSNYAAWEV AEICTLCKSN GWILPTVYQG MYNAITRQVE TELFPCLRHF GLRFYAFNPL AGGLLTGKYK YEDKDGKQPV GRFFGNTWAE MYRNRYWKEH HFEGIALVEK ALQAAYGASA PSMTSATLRW MYHHSQLQGA HGDAVILGMS SLEQLEQNLA AAEEGPLEPA VVDAFNQAWH LVAHECPNYF R
Product Science Overview
Aldo-Keto Reductase Family 7 Member A3 (AKR7A3) is a protein encoded by the AKR7A3 gene in humans. This protein is part of the aldo-keto reductase (AKR) superfamily, which consists of more than 40 known enzymes and proteins . These enzymes are involved in the detoxification of aldehydes and ketones, playing a crucial role in cellular metabolism and protection against toxic substances .
The AKR7A3 gene is located on chromosome 1p36.13 and spans approximately 6.2 kb of genomic DNA . The gene encodes a protein that is primarily expressed in the liver, but also found in other tissues such as the pancreas, kidney, and stomach . The protein consists of 323 amino acids and has a molecular weight of approximately 37 kDa .
AKR7A3 is known for its role in the detoxification of aflatoxin B1 (AFB1), a potent hepatocarcinogen. The enzyme reduces the dialdehyde protein-binding form of AFB1 to the non-binding AFB1 dialcohol, thereby preventing its toxic and carcinogenic effects . This detoxification process is crucial for protecting the liver from damage caused by aflatoxins, which are common contaminants in food and feed .
In addition to its role in aflatoxin detoxification, AKR7A3 is involved in the metabolism of other aldehydes and ketones, contributing to the overall cellular defense against oxidative stress and toxic compounds .
Mutations or alterations in the expression of the AKR7A3 gene have been associated with various diseases, including congenital symmetric circumferential skin creases and multiple benign circumferential skin creases on limbs . The enzyme’s role in detoxifying aflatoxins also highlights its potential importance in preventing liver cancer and other liver-related diseases .
Recombinant AKR7A3, expressed in E. coli, is widely used in research to study its enzymatic properties and potential therapeutic applications . The recombinant protein is typically purified to high levels of purity (≥95%) and is used in various biochemical assays to understand its function and mechanism of action .
