AK2 Mouse

In vertebrates, there are three main isoforms of adenylate kinase: AK1, AK2, and AK3. These isoforms are tissue-specific and developmentally regulated. AK2 is primarily localized in the mitochondrial intermembrane space and is involved in apoptosis . This localization is crucial for its role in maintaining the balance of adenine nucleotides within the mitochondria, which is essential for cellular energy metabolism and signaling.

The recombinant form of AK2 from mice is produced in Escherichia coli (E. coli). It is a single, non-glycosylated polypeptide chain containing 263 amino acids and has a molecular mass of approximately 29 kDa . The recombinant protein is often fused to a His-tag at the N-terminus to facilitate purification through chromatographic techniques.

AK2’s specific activity is measured by its ability to convert ADP to ATP and AMP. The recombinant mouse AK2 has a specific activity greater than 40 units/mg, where one unit will convert 2.0 micromoles of ADP to ATP and AMP per minute at pH 7.5 and 37°C .

Mutations in the AK2 gene are associated with reticular dysgenesis, a severe combined immunodeficiency characterized by a lack of white blood cells and sensorineural hearing loss . The enzyme’s role in apoptosis and energy metabolism makes it a potential target for therapeutic interventions in various diseases, including neurodegenerative disorders and metabolic syndromes .

The recombinant AK2 protein is stable at 4°C for up to one week but should be stored below -18°C for long-term preservation. It is important to avoid freeze-thaw cycles to maintain its stability and activity .