AK2 Antibody

Adenylate Kinase 2, Mouse Anti Human
Cat. No.
BT13264
Source
Synonyms
ADK2, AK-2, Adenylate kinase isoenzyme 2 mitochondrial, ATP-AMP transphosphorylase 2, adenylate kinase 2.
Appearance
Sterile filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

Product Specs

Introduction
Adenylate kinases are enzymes that help regulate the balance of adenine nucleotides within cells. They do this by facilitating the reversible transfer of phosphate groups among these nucleotides. In vertebrates, there are three main types of adenylate kinase isozymes: AK1, AK2, and AK3. Each isozyme exhibits specific tissue distribution and is regulated during development. AK2 is particularly localized within the mitochondrial intermembrane space and plays a crucial role in apoptosis, a programmed cell death process. Mutations in the AK2 gene have been linked to a condition called reticular dysgenesis.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The solution contains 1mg/ml of the antibody in a buffer composed of PBS at pH 7.4, 10% Glycerol, and 0.02% Sodium Azide.
Storage Procedures
For short-term storage (up to 1 month), keep the antibody at 4°C. For long-term storage, it is recommended to store at -20°C. Avoid repeated freeze-thaw cycles to maintain stability.
Stability / Shelf Life
The antibody remains stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
This antibody has undergone rigorous testing in various applications, including ELISA, Western blot analysis, Flow cytometry, and ICC/IF, demonstrating its specificity and reactivity. However, it is essential to optimize the antibody concentration for each specific application to achieve the best results.
Synonyms
ADK2, AK-2, Adenylate kinase isoenzyme 2 mitochondrial, ATP-AMP transphosphorylase 2, adenylate kinase 2.
Purification Method
AK2 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT7E7AT.
Immunogen
Anti-human AK2 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human AK2 protein 1-239 amino acids purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and k light chain.

Product Science Overview

Introduction

Adenylate kinase 2 (AK2) is an enzyme that plays a crucial role in cellular energy homeostasis by catalyzing the interconversion of adenine nucleotides. Specifically, it facilitates the reaction: ATP + AMP ⇔ 2 ADP. This enzyme is part of the nucleoside monophosphate (NMP) kinase family, which is essential for nucleotide metabolism .

Structure and Function

Adenylate kinase 2 is a mitochondrial isoenzyme, meaning it is localized within the mitochondria of cells. It is involved in maintaining the balance of adenine nucleotides within the cell, which is vital for various cellular processes, including energy transfer and signal transduction . The enzyme’s activity ensures that the levels of ATP, ADP, and AMP are regulated, which is critical for cellular energy homeostasis .

Isoforms and Localization

There are multiple isoforms of adenylate kinase, each with specific functions and localizations within the cell. AK2 is primarily found in the mitochondria, whereas other isoforms, such as AK1, are found in the cytosol. The different isoforms have varying affinities for their substrates and are involved in distinct cellular processes .

Medical Relevance

Mutations or dysregulation of adenylate kinase 2 have been linked to several medical conditions. For instance, AK2 mutations are associated with reticular dysgenesis, a severe form of immunodeficiency characterized by a lack of white blood cells and sensorineural hearing loss . Additionally, the impairment of mitochondrial isoenzymes like AK2 has been demonstrated in neuroblastoma and glioma, highlighting its role in various diseases .

Mouse Anti Human AK2 Antibody

The mouse anti-human AK2 antibody is a monoclonal antibody used in research to study the expression and function of AK2 in human cells. This antibody is produced by immunizing mice with human AK2 protein, followed by the isolation and cloning of specific B cells that produce the desired antibody. The resulting monoclonal antibody can be used in various applications, including Western blotting, immunohistochemistry, and flow cytometry, to detect and quantify AK2 in human samples .

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