AK1 Mouse
Adenylate kinase Isoenzyme 1 isoform 1, Ak-1, B430205N08Rik, ATP-AMP transphosphorylase 1, ATP:AMP phosphotransferase, Adenylate monophosphate kinase, Myokinase.
Greater than 90.0% as determined by SDS-PAGE.
Description
AK1 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 233 amino acids (1-210 a.a) and having a molecular mass of 25.5kDa.
AK1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Adenylate kinase Isoenzyme 1 isoform 1, Ak-1, B430205N08Rik, ATP-AMP transphosphorylase 1, ATP:AMP phosphotransferase, Adenylate monophosphate kinase, Myokinase.
MGSSHHHHHH SSGLVPRGSH MGSMGCCVSS EPQEEGGRKT GEKLKKAKII FVVGGPGSGK GTQCEKIVQK YGYTHLSTGD LLRAEVSSGS ERGKKLSAIM EKGELVPLDT VLDMLRDAML AKVDSSNGFL IDGYPREVKQ GEEFEQKIGQ PTLLLYVDAG AETMTQRLLK RGETSGRVDD
NEETIKKRLE TYYNATEPVI SFYDKRGIVR KVNAEGTVDT VFSEVCTYLD SLK.
Product Science Overview
Adenylate Kinase 1 (AK1) is a small, ubiquitous enzyme that plays a crucial role in cellular energy homeostasis. It belongs to the nucleoside monophosphate (NMP) kinase family, which are key enzymes in nucleotide metabolism. AK1 catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP, a reaction essential for maintaining the balance of adenine nucleotides within the cell.
The mouse recombinant AK1 is produced in Escherichia coli and is a single, non-glycosylated polypeptide chain containing 233 amino acids, with a molecular mass of approximately 25.5 kDa . The enzyme is fused to a 23 amino acid His-tag at the N-terminus, which facilitates its purification through chromatographic techniques .
AK1 is primarily found in the cytosol of skeletal muscle, brain, and erythrocytes. It is essential for cell growth and maintenance, as it regulates the adenine nucleotide composition within the cell. The enzyme’s activity ensures a rapid response to changes in cellular energy demands by maintaining the balance between ATP, ADP, and AMP levels .
Defects in the AK1 gene can lead to various medical conditions. For instance, mutations in AK1 are associated with a form of hemolytic anemia, a condition characterized by the premature destruction of red blood cells . Additionally, dysregulation or mutation of adenylate kinases, in general, has been linked to several diseases, including metabolic syndrome and neurodegenerative disorders .
Due to its critical role in energy metabolism, AK1 is a valuable target for research and pharmaceutical development. The enzyme’s activity can be measured to assess cellular energy status, and its dysregulation can serve as a marker for early diagnosis of various diseases . Recombinant AK1 is used in laboratory research to study its biochemical properties, structure-function relationships, and potential therapeutic applications.
