AK1 Antibody

Adenylate Kinase 1 (AK1) is an enzyme that plays a crucial role in cellular energy homeostasis by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. This enzyme is essential for maintaining the balance of adenosine triphosphate (ATP), adenosine diphosphate (ADP), and adenosine monophosphate (AMP) within cells.

AK1 is one of the three isozymes of adenylate kinase identified in vertebrates, the others being AK2 and AK3. AK1 is predominantly found in the cytosol of skeletal muscle, brain, and erythrocytes. It facilitates the interconversion of ATP and AMP to two molecules of ADP, a reaction that is vital for cellular energy transfer and metabolic regulation.

The AK1 gene has been associated with a rare genetic disorder causing nonspherocytic hemolytic anemia. Mutations in the AK1 gene can lead to reduced catalytic activity of the enzyme, resulting in impaired energy metabolism and various clinical manifestations .

Mouse anti human AK1 antibodies are monoclonal or polyclonal antibodies developed in mice to target and bind specifically to human AK1. These antibodies are widely used in scientific research for various applications, including:

• Western Blot: To detect and quantify AK1 protein levels in different samples.
• Immunocytochemistry: To visualize the localization of AK1 within cells.
• ELISA: To measure AK1 concentrations in biological fluids.
• Flow Cytometry: To analyze the expression of AK1 on the cell surface.
• Immunohistochemistry: To study the distribution of AK1 in tissue sections .

The use of mouse anti human AK1 antibodies has significantly advanced our understanding of AK1’s role in cellular metabolism and its implications in various diseases. These antibodies have been validated for specificity and are available in different formats to suit various experimental needs .