AHCY Mouse
EC 3.3.1.1, SAHH, AdoHcyase, S-adenosyl-L-homocysteine hydrolase, Adenosylhomocysteinase.
Sterile filtered colorless solution.
Greater than 90.0% as determined by SDS-PAGE.
Description
AHCY Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 456 amino acids (1-432 a.a) and having a molecular mass of 50.2kDa.
AHCY is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
EC 3.3.1.1, SAHH, AdoHcyase, S-adenosyl-L-homocysteine hydrolase, Adenosylhomocysteinase.
MGSSHHHHHH SSGLVPRGSH MGSHMSDKLP YKVADIGLAA WGRKALDIAE NEMPGLMRMR EMYSASKPLK GARIAGCLHM TVETAVLIET LVALGAEVRW SSCNIFSTQD HAAAAIAKAG IPVFAWKGET DEEYLWCIEQ TLHFKDGPLN MILDDGGDLT NLIHTKYPQL LSGIRGISEE TTTGVHNLYK MMSNGILKVP AINVNDSVTK SKFDNLYGCR ESLIDGIKRA TDVMIAGKVA VVAGYGDVGK GCAQALRGFG ARVIITEIDP INALQAAMEG YEVTTMDEAC KEGNIFVTTT GCVDIILGRH FEQMKDDAIV CNIGHFDVEI DVKWLNENAV EKVNIKPQVD RYWLKNGRRI ILLAEGRLVN LGCAMGHPSF VMSNSFTNQV MAQIELWTHP DKYPVGVHFL PKKLDEAVAE AHLGKLNVKL TKLTEKQAQY LGMPINGPFK PDHYRY.
Product Science Overview
Adenosylhomocysteinase (AHCY), also known as S-adenosyl-L-homocysteine hydrolase (SAHH), is a highly conserved enzyme found in various organisms, including bacteria, yeast, plants, and mammals . In mammals, AHCY plays a crucial role in the methionine cycle, a vital metabolic pathway involved in the transfer of one-carbon units for biosynthetic processes, amino acid homeostasis, redox cellular control, and epigenetic regulation .
AHCY catalyzes the reversible hydrolysis of S-adenosylhomocysteine (SAH) into adenosine (Ado) and homocysteine (Hcy) . This reaction is essential because SAH is a potent inhibitor of methyltransferases, enzymes that transfer methyl groups from S-adenosylmethionine (SAM) to various substrates, including nucleic acids and proteins . By breaking down SAH, AHCY ensures the proper functioning of methyltransferases and maintains the balance of methylation reactions within the cell .
Recombinant mouse AHCY is a form of the enzyme produced through recombinant DNA technology, typically expressed in E. coli . This recombinant protein is often tagged with a His-tag at the N-terminus to facilitate purification and detection . The recombinant mouse AHCY consists of 432 amino acids and has a molecular weight of approximately 50.2 kDa .
Recombinant mouse AHCY is widely used in research to study the enzyme’s structure, function, and role in various biological processes. It is also employed in assays to investigate the effects of different compounds on AHCY activity and to understand the enzyme’s involvement in diseases related to methionine metabolism .
AHCY deficiency in humans is associated with a rare recessive disorder that affects methionine metabolism . This condition can lead to various symptoms, including developmental delays, muscle weakness, and liver abnormalities . Understanding the function and regulation of AHCY is crucial for developing potential therapeutic strategies for such metabolic disorders.
