AHCY Human, Sf9
Sf9, Baculovirus cells.
EC 3.3.1.1, SAHH, AdoHcyase, S-adenosyl-L-homocysteine hydrolase, AHCY, Adenosylhomocysteinase.
Greater than 90% as determined by SDS-PAGE.
Description
AHCY Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 441 amino acids (1-432 a.a.) and having a molecular mass of 48.8kDa (Migrates at 40-57kDa on SDS-PAGE under reducing conditions). AHCY is fused to a 6 amino acids His-Tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
EC 3.3.1.1, SAHH, AdoHcyase, S-adenosyl-L-homocysteine hydrolase, AHCY, Adenosylhomocysteinase.
Sf9, Baculovirus cells.
ADLMSDKLPY KVADIGLAAW GRKALDIAEN EMPGLMRMRE RYSASKPLKG ARIAGCLHMT VETAVLIETL VTLGAEVQWS SCNIFSTQDH AAAAIAKAGI PVYAWKGETD EEYLWCIEQT LYFKDGPLNM ILDDGGDLTN LIHTKYPQLL PGIRGISEET TTGVHNLYKM MANGILKVPA INVNDSVTKS KFDNLYGCRE SLIDGIKRAT DVMIAGKVAV VAGYGDVGKG CAQALRGFGA RVIITEIDPI NALQAAMEGY EVTTMDEACQ EGNIFVTTTG CIDIILGRHF EQMKDDAIVC NIGHFDVEID VKWLNENAVE KVNIKPQVDR YRLKNGRRII LLAEGRLVNL GCAMGHPSFV MSNSFTNQVM AQIELWTHPD KYPVGVHFLP KKLDEAVAEA HLGKLNVKLT KLTEKQAQYL GMSCDGPFKP DHYRYHHHHH H.
Product Science Overview
Adenosylhomocysteinase (AHCY) is a crucial enzyme involved in the metabolism of S-adenosylhomocysteine (SAH). The recombinant form of this enzyme, produced in Sf9 Baculovirus cells, is widely used in research to study its function and role in various biological processes.
The human recombinant AHCY produced in Sf9 cells is a single, glycosylated polypeptide chain containing 441 amino acids, with a molecular mass of approximately 48.8 kDa . This enzyme is fused to a 6-amino acid His-Tag at the C-terminus, which facilitates its purification through chromatographic techniques .
AHCY catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) into adenosine (Ado) and L-homocysteine (Hcy) . This reaction is crucial for maintaining the intracellular concentration of SAH, which is essential for transmethylation reactions . Transmethylation is a vital process in which methyl groups are transferred from one molecule to another, playing a significant role in DNA methylation, protein function, and lipid metabolism .
The regulation of SAH levels by AHCY is critical for various cellular processes. Methylation, controlled by AHCY, influences gene expression, protein function, and signal transduction . A deficiency in AHCY can lead to hypermethioninemia, a condition characterized by elevated levels of methionine in the blood .
The recombinant form of AHCY produced in Sf9 cells is used extensively in laboratory research. It allows scientists to study the enzyme’s structure, function, and interactions in a controlled environment. This research can lead to a better understanding of metabolic disorders and the development of potential therapeutic interventions .
AHCY (Human Recombinant, Sf9) is typically stored at 4°C for short-term use (2-4 weeks) and at -20°C for long-term storage. It is recommended to add a carrier protein, such as 0.1% HSA or BSA, to prevent degradation during storage . The enzyme should be handled carefully to avoid multiple freeze-thaw cycles, which can affect its stability and activity .
