AFP Antibody
Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, AFP, FETA, HPAFP.
Description
Product Specs
Alpha-fetoprotein (AFP) is typically produced in the liver, digestive system, and the yolk sac during fetal development. Antibodies targeting AFP have proven valuable in identifying hepatocellular carcinomas (HCC) and germ cell tumors, particularly yolk sac tumors.
The solution has a concentration of 1mg/ml and contains PBS with a pH of 7.4, 10% Glycerol, and 0.02% Sodium Azide.
The AFP antibody has undergone testing using ELISA and Western blot analysis to ensure its specificity and reactivity. However, due to variations in applications, it is recommended to titrate the reagent for each specific use to achieve optimal outcomes.
Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, AFP, FETA, HPAFP.
AFP antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
PAT73E10AT.
Anti-human AFP mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human AFP protein 19-609 amino acids purified from insect cell.
Mouse IgG1 heavy chain and k light chain.
Product Science Overview
Alpha-fetoprotein (AFP) is a major plasma protein produced by the yolk sac and liver during fetal development. It is similar to serum albumin and binds and transports various ligands such as nickel, copper, bilirubin, and fatty acids . In adults, AFP levels are typically low to absent, but elevated levels can indicate certain malignancies, such as hepatocellular carcinoma or teratoblastoma .
Mouse Anti-Human Alpha Fetoprotein antibodies are monoclonal antibodies derived from mouse myeloma cells and splenocytes from BALB/c mice immunized with purified human AFP . These antibodies are highly specific and are used in various research and diagnostic applications, including:
- Western Blotting: Detects AFP in cell lysates, such as those from HepG2 human hepatocellular carcinoma cell lines .
- Immunocytochemistry: Used to stain AFP in fixed cells, providing insights into the localization of AFP within the cytoplasm .
- Flow Cytometry: Utilized for intracellular staining to detect AFP in cell populations .
The use of Mouse Anti-Human AFP antibodies is crucial in both research and clinical diagnostics. Here are some key applications:
- Cancer Research: AFP is an oncofetal antigen, meaning it is expressed during fetal development and re-expressed in certain cancers. Researchers use these antibodies to study the expression patterns of AFP in cancer cells, aiding in the understanding of tumor biology and the development of targeted therapies .
- Diagnostic Testing: Elevated AFP levels in adults can be an early marker of liver cancer or germ cell tumors. Mouse Anti-Human AFP antibodies are used in diagnostic assays to measure AFP levels in patient samples, helping in early detection and monitoring of these cancers .
- Developmental Biology: Studying AFP expression during fetal development provides insights into normal and abnormal developmental processes. These antibodies help researchers track AFP levels and distribution in developing tissues .
