ADRM1 Human

Adhesion Regulating Molecule 1 Human Recombinant
Cat. No.
BT22882
Source
Escherichia Coli.
Synonyms
Proteasomal ubiquitin receptor ADRM1, 110 kDa cell membrane glycoprotein, Gp110, Adhesion-regulating molecule 1, ARM-1, Proteasome regulatory particle non-ATPase 13, hRpn13, Rpn13 homolog, ADRM1, ARM1, Rpn13.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

ADRM1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 431 amino acids (1-407 a.a) and having a molecular mass of 44.7kDa.
ADRM1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The proteasomal ubiquitin receptor ADRM1 (ADRM1) acts as a receptor for ubiquitin at the proteasome. This integral plasma membrane protein is involved in cell adhesion. ADRM1 is believed to be modified by O-linked glycosylation. It interacts with and enhances the activity of the deubiquitinating enzyme UCHL5 at the 26S proteasome.
Description
Recombinant human ADRM1, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 431 amino acids (residues 1-407). It has a molecular weight of 44.7 kDa. The protein includes a 24-amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The ADRM1 protein is supplied in a solution at a concentration of 1 mg/ml. The solution is buffered with 20 mM Tris-HCl (pH 8.0) and contains 20% glycerol, 0.1 M NaCl, and 1 mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the protein can be stored at 4°C. For extended storage, it is recommended to freeze the protein at -20°C. To ensure long-term stability during frozen storage, adding a carrier protein such as 0.1% HSA or BSA is advised. Repeated freezing and thawing of the protein should be avoided.
Purity
The purity of the ADRM1 protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
Proteasomal ubiquitin receptor ADRM1, 110 kDa cell membrane glycoprotein, Gp110, Adhesion-regulating molecule 1, ARM-1, Proteasome regulatory particle non-ATPase 13, hRpn13, Rpn13 homolog, ADRM1, ARM1, Rpn13.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMTTSGA LFPSLVPGSR GASNKYLVEF RAGKMSLKGT TVTPDKRKGL VYIQQTDDSL IHFCWKDRTS GNVEDDLIIF PDDCEFKRVP QCPSGRVYVL KFKAGSKRLF FWMQEPKTDQ DEEHCRKVNE YLNNPPMPGA LGASGSSGHE LSALGGEGGL QSLLGNMSHS QLMQLIGPAG LGGLGGLGAL TGPGLASLLG SSGPPGSSSS SSSRSQSAAV TPSSTTSSTR ATPAPSAPAA ASATSPSPAP SSGNGASTAA SPTQPIQLSD LQSILATMNV PAGPAGGQQV DLASVLTPEI MAPILANADV QERLLPYLPS GESLPQTADE IQNTLTSPQF QQALGMFSAA LASGQLGPLM CQFGLPAEAV EAANKGDVEA FAKAMQNNAK PEQKEGDTKD KKDEEEDMSL D.

Product Science Overview

Structure and Function

ADRM1 is a component of the 26S proteasome, a large protein complex responsible for the ATP-dependent degradation of ubiquitinated proteins . The proteasome is essential for maintaining cellular homeostasis by removing misfolded or damaged proteins and proteins that are no longer needed. ADRM1 acts as a ubiquitin receptor within the proteasome, recruiting the deubiquitinating enzyme ubiquitin carboxyl-terminal hydrolase L5 (UCHL5) . This interaction is vital for the regulation of protein degradation.

Role in Cell Adhesion

Although ADRM1 is primarily an intracellular protein, its increased levels are associated with enhanced cell adhesion . This effect is likely indirect and may be mediated through its role in protein degradation and signal transduction pathways. Dysregulation of ADRM1 has been implicated in various pathological conditions, including cancer.

Implications in Cancer

ADRM1 has been identified as a potential biomarker and therapeutic target in several types of cancer, including bladder cancer . Studies have shown that ADRM1 expression is significantly elevated in bladder cancer tissues compared to adjacent normal tissues . High ADRM1 expression is associated with poor overall survival in bladder cancer patients . Functional analyses have revealed that ADRM1 is involved in immune-related pathways and is positively correlated with key immune checkpoints such as CD274 (PD-L1), PDCD1 (PD-1), and PDCD1LG2 (PD-L2) . This suggests that ADRM1 may play a role in the tumor microenvironment and could be a target for immunotherapy.

Therapeutic Potential

The prognostic significance of ADRM1 in cancer patients highlights its potential as a therapeutic target. Patients with high ADRM1 expression may benefit from immunotherapy, while those with low ADRM1 expression may be more sensitive to traditional chemotherapy drugs such as cisplatin, docetaxel, vinblastine, mitomycin C, and methotrexate . This dual role underscores the importance of ADRM1 in personalized cancer treatment strategies.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

ADRM1 Antibody
Adhesion Regulating Molecule 1, Mouse Anti Human
Cat. No.
BT11585
Source
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.