ADK Human

Adenosine Kinase Human Recombinant
Cat. No.
BT25186
Source
Escherichia Coli.
Synonyms
Adenosine 5'-phosphotransferase, EC 2.7.1.20, AK, ADK.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Adenosine Kinase Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 362 amino acids (22-362a.a.) and having a molecular mass of 40.5 kDa. Adenosine Kinase protein is fused to a 21 amino acid His tag at N-terminus and is purified by standard chromatography.

Product Specs

Introduction
Adenosine Kinase is a key enzyme found in mammalian tissues. It plays a vital role in regulating levels of adenosine and adenine nucleotides by transferring a phosphate group from ATP to adenosine. This function makes it important for the cardiovascular, nervous, respiratory, and immune systems. Inhibiting this enzyme is a significant pharmacological strategy to increase adenosine levels in blood vessels and reduce inflammation.
Description
Recombinant Human Adenosine Kinase, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 362 amino acids (specifically, residues 22-362). It has a molecular weight of 40.5 kDa. A 21 amino acid His tag is fused to the N-terminus of the protein, which is then purified using standard chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The Adenosine Kinase protein is supplied in a solution at a concentration of 0.5 mg/ml. The solution also contains 20mM Tris-HCl buffer at pH 8, 1mM DTT, 1mM EDTA, 50mM NaCl, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), keep the solution refrigerated at 4°C. For longer periods, freeze the solution at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is recommended for long-term storage. Avoid repeated freezing and thawing.
Purity
The purity is determined to be greater than 95% using SDS-PAGE analysis.
Synonyms
Adenosine 5'-phosphotransferase, EC 2.7.1.20, AK, ADK.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MRENILFGMG NPLLDISAVV DKDFLDKYSL KPNDQILAED KHKELFDELV KKFKVEYHAG GSTQNSIKVA QWMIQQPHKA ATFFGCIGID KFGEILKRKA AEAHVDAHYY EQNEQPTGTC AACITGDNRS LIANLAAANC YKKEKHLDLE KNWMLVEKAR VCYIAGFFLT VSPESVLKVA HHASENNRIF TLNLSAPFIS QFYKESLMKV MPYVDILFGN ETEAATFARE QGFETKDIKE IAKKTQALPK MNSKRQRIVI FTQGRDDTIM ATESEVTAFA VLDQDQKEII DTNGAGDAFV GGFLSQLVSD KPLTECIRAG HYAASIIIRR TGCTFPEKPD FH.

Product Science Overview

Introduction

Adenosine kinase (ADK) is a crucial enzyme in cellular metabolism, playing a significant role in the regulation of adenosine levels within the cell. Adenosine itself is a vital molecule involved in various physiological processes, including energy transfer, signal transduction, and the regulation of blood flow. The recombinant form of human adenosine kinase is produced using advanced biotechnological methods, allowing for its use in research and therapeutic applications.

Structure and Function

Adenosine kinase is an enzyme that catalyzes the phosphorylation of adenosine to form adenosine monophosphate (AMP). This reaction is essential for maintaining the balance of adenosine within the cell, as adenosine levels can influence numerous cellular functions. The enzyme is encoded by the ADK gene and is highly conserved across different species, highlighting its importance in cellular physiology .

The recombinant human adenosine kinase is typically produced in E. coli and purified to high levels of purity, often exceeding 95% as determined by SDS-PAGE analysis . The enzyme is usually tagged with a histidine tag to facilitate purification and detection.

Biological Significance

Adenosine kinase plays a pivotal role in the regulation of extracellular adenosine concentrations. By converting adenosine to AMP, ADK helps to modulate the availability of adenosine for receptor-mediated signaling pathways. This regulation is crucial for various physiological processes, including:

  • Energy Metabolism: Adenosine is a key component of ATP, the primary energy currency of the cell. By regulating adenosine levels, ADK indirectly influences cellular energy metabolism.
  • Neuroprotection: Adenosine has neuroprotective properties, and its regulation by ADK is important in conditions such as ischemia and epilepsy.
  • Cardiovascular Function: Adenosine is involved in the regulation of blood flow and cardiac function. ADK helps to maintain appropriate levels of adenosine, ensuring proper cardiovascular responses.
Applications in Research and Medicine

Recombinant human adenosine kinase is widely used in research to study its role in various physiological and pathological conditions. Some key applications include:

  • Drug Development: ADK inhibitors are being explored as potential therapeutic agents for conditions such as epilepsy, pain, and inflammation. Recombinant ADK is used to screen and characterize these inhibitors.
  • Metabolic Studies: Researchers use recombinant ADK to investigate the metabolic pathways involving adenosine and its derivatives.
  • Neuroscience Research: The enzyme is used to study the role of adenosine in neuroprotection and neurodegenerative diseases.

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THE BIOTEK
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