ADK Antibody
Description
Product Specs
Product Science Overview
Adenosine kinase (AdK) is an enzyme that plays a crucial role in cellular metabolism by catalyzing the phosphorylation of adenosine to adenosine monophosphate (AMP) using adenosine triphosphate (ATP) as the phosphate donor . This reaction is essential for maintaining the balance of adenosine levels within cells, which is important for various physiological processes, including energy metabolism, signal transduction, and nucleic acid synthesis.
In humans, adenosine kinase is widely expressed in various tissues, including the liver, brain, and heart. It is involved in regulating the extracellular levels of adenosine, a molecule that has significant effects on cardiovascular function, immune response, and neural activity . Elevated levels of adenosine can have protective effects during stress conditions such as hypoxia and inflammation, making adenosine kinase a potential therapeutic target for various diseases .
Mouse anti human adenosine kinase antibodies are monoclonal antibodies produced by immunizing mice with human adenosine kinase. These antibodies are highly specific and are used in various research applications, including enzyme-linked immunosorbent assays (ELISA), western blotting, and immunohistochemistry. They help in detecting and quantifying adenosine kinase in human tissues and cells, providing valuable insights into its role in health and disease.
The preparation of mouse anti human adenosine kinase antibodies involves several steps:
- Immunization: Mice are immunized with purified human adenosine kinase protein to elicit an immune response.
- Hybridoma Production: Spleen cells from the immunized mice are fused with myeloma cells to create hybridoma cells that can produce monoclonal antibodies.
- Screening and Selection: Hybridoma cells are screened for the production of antibodies that specifically bind to human adenosine kinase. Positive clones are selected and expanded.
- Purification: The monoclonal antibodies are purified from the culture supernatant using techniques such as protein A/G affinity chromatography.
Mouse anti human adenosine kinase antibodies are valuable tools in biomedical research. They are used to study the expression and regulation of adenosine kinase in various tissues and under different physiological and pathological conditions. These antibodies can also be used to investigate the role of adenosine kinase in diseases such as cancer, cardiovascular diseases, and neurological disorders .
