ACSF2 Human

Acyl-CoA Synthetase Family Member 2 Human Recombinant
Cat. No.
BT25575
Source
E.coli.
Synonyms
Acyl-CoA Synthetase Family Member 2, PPARG Binding, Long Chain Fatty Acid Acyl Co-A Ligase Like, Acyl-CoA Synthetase Family Member 2, Mitochondrial, EC 6.2.1.26, EC 6.2.1.-, FLJ20920, EC 6.2.1, AVYV493, ACSMW, Acyl-CoA synthetase family member 2, mitochondrial.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ACSF2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 597 amino acids (42-615 a.a) and having a molecular mass of 66.1kDa.
ACSF2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
ACSF2, or Acyl-CoA synthetase family member 2, belongs to the ATP-dependent AMP-binding enzyme family. Acyl-CoA synthetases catalyze the thioesterification of fatty acids with coenzyme A, generating activated intermediates essential for lipid metabolism and homeostasis. ACSF2 is crucial for lipid synthesis, beta-oxidation for energy production, protein acylation, and fatty-acid dependent transcriptional regulation. It also plays a vital role in the vectorial acylation process, facilitating fatty acid import into cells.
Description
Recombinant Human ACSF2, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 597 amino acids (42-615 a.a), with a molecular weight of 66.1 kDa. The protein includes a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The ACSF2 protein solution is provided at a concentration of 0.5 mg/ml. It is formulated in Phosphate Buffered Saline (PBS) at pH 7.4 and contains 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, freezing at -20°C is recommended. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
Purity is determined by SDS-PAGE analysis and exceeds 95%.
Synonyms
Acyl-CoA Synthetase Family Member 2, PPARG Binding, Long Chain Fatty Acid Acyl Co-A Ligase Like, Acyl-CoA Synthetase Family Member 2, Mitochondrial, EC 6.2.1.26, EC 6.2.1.-, FLJ20920, EC 6.2.1, AVYV493, ACSMW, Acyl-CoA synthetase family member 2, mitochondrial.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSLSSREVD RMVSTPIGGL SYVQGCTKKH LNSKTVGQCL ETTAQRVPER EALVVLHEDV RLTFAQLKEE VDKAASGLLS IGLCKGDRLG MWGPNSYAWV LMQLATAQAG IILVSVNPAY QAMELEYVLK KVGCKALVFP KQFKTQQYYN VLKQICPEVE NAQPGALKSQ RLPDLTTVIS VDAPLPGTLL LDEVVAAGST RQHLDQLQYN QQFLSCHDPI NIQFTSGTTG SPKGATLSHY NIVNNSNILG ERLKLHEKTP EQLRMILPNP LYHCLGSVAG TMMCLMYGAT LILASPIFNG KKALEAISRE RGTFLYGTPT MFVDILNQPD FSSYDISTMC GGVIAGSPAP PELIRAIINK INMKDLVVAY GTTENSPVTF AHFPEDTVEQ KAESVGRIMP HTEARIMNME AGTLAKLNTP GELCIRGYCV MLGYWGEPQK TEEAVDQDKW YWTGDVATMN EQGFCKIVGR SKDMIIRGGE NIYPAELEDF FHTHPKVQEV QVVGVKDDRM GEEICACIRL KDGEETTVEE IKAFCKGKIS HFKIPKYIVF VTNYPLTISG KIQKFKLREQ MERHLNL.

Product Science Overview

Structure and Function

ACSF2 is a mitochondrial enzyme that exhibits medium-chain fatty acid-CoA ligase activity . This means it has a preference for medium-chain fatty acids as substrates. The enzyme’s primary function is to activate fatty acids by converting them into acyl-CoA, which can then enter various metabolic pathways, including β-oxidation and lipid biosynthesis .

Role in Metabolism

ACSF2 is integral to the fatty acid metabolic process. By forming acyl-CoA thioesters, it facilitates the breakdown and utilization of fatty acids for energy production. This process is essential for maintaining cellular energy homeostasis, especially in tissues with high metabolic demands .

Clinical Significance

Mutations or dysregulation of the ACSF2 gene can have significant implications for human health. For instance, abnormalities in fatty acid metabolism can lead to metabolic disorders, including obesity, diabetes, and fatty liver disease. Additionally, ACSF2 has been implicated in the differentiation of adipocytes, the cells responsible for storing fat .

Research and Therapeutic Potential

Given its central role in fatty acid metabolism, ACSF2 is a potential target for therapeutic interventions aimed at treating metabolic disorders. Research is ongoing to better understand the enzyme’s function and regulation, which could lead to the development of novel treatments for conditions related to fatty acid metabolism .

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THE BIOTEK
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