ACSF2 Antibody

Acyl-CoA Synthetase Family Member 2, Mouse Anti Human
Cat. No.
BT12681
Source
Synonyms

Acyl-CoA Synthetase Family Member 2, PPARG Binding, Long Chain Fatty Acid Acyl Co-A Ligase Like, Acyl-CoA Synthetase Family Member 2, Mitochondrial, EC 6.2.1.26, EC 6.2.1.-, FLJ20920, EC 6.2.1, AVYV493, ACSMW, Acyl-CoA synthetase family member 2, mitochondrial.

Appearance
Sterile filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

Product Specs

Introduction
Acyl-CoA synthetase family member 2, also known as ACSF2, is an enzyme that plays a crucial role in lipid metabolism. It belongs to the ATP-dependent AMP-binding enzyme family and is responsible for activating fatty acids by converting them into acyl-CoA esters. These activated intermediates are essential for various cellular processes, including lipid synthesis, energy production through beta-oxidation, protein modification, and the regulation of gene expression influenced by fatty acids. ACSF2 also facilitates the uptake of fatty acids into cells through a mechanism called vectorial acylation.
Physical Appearance
A clear and colorless liquid that has been sterilized by filtration.
Formulation
This solution contains 1mg of ACSF2 antibody per milliliter (1mg/ml) in a buffer solution consisting of phosphate-buffered saline (PBS) at a pH of 7.4, 10% glycerol, and 0.02% sodium azide.
Storage Procedures
For short-term storage (up to 1 month), keep the solution refrigerated at 4°C. For longer storage durations, it is recommended to store the solution at -20°C. Repeated freezing and thawing of the solution should be avoided.
Stability / Shelf Life
The product has a shelf life of 12 months when stored at -20°C and remains stable for 1 month at 4°C.
Applications
The ACSF2 antibody has undergone rigorous testing using various methods such as ELISA, Western blot analysis, and immunocytochemistry/immunofluorescence (ICC/IF). These tests have confirmed its specificity and reactivity, ensuring its reliability for detecting and analyzing ACSF2. However, it's important to note that optimal working concentrations may vary depending on the specific application and experimental conditions. Therefore, it is recommended to perform titration experiments to determine the optimal reagent concentration for each specific use.
Synonyms

Acyl-CoA Synthetase Family Member 2, PPARG Binding, Long Chain Fatty Acid Acyl Co-A Ligase Like, Acyl-CoA Synthetase Family Member 2, Mitochondrial, EC 6.2.1.26, EC 6.2.1.-, FLJ20920, EC 6.2.1, AVYV493, ACSMW, Acyl-CoA synthetase family member 2, mitochondrial.

Purification Method

ACSF2 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.

Type
Mouse Anti Human Monoclonal.
Clone

PAT18B4AT.

Immunogen

Anti-human ACSF2 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human ACSF2 amino acids 42-615 purified from E. coli.

Ig Subclass

Mouse IgG1 heavy chain and κ light chain.

Product Science Overview

Introduction

Acyl-CoA Synthetase Family Member 2 (ACSF2) is a protein-coding gene that plays a crucial role in fatty acid metabolism. This gene is part of the acyl-CoA synthetase family, which is responsible for catalyzing the initial reaction in fatty acid metabolism by forming a thioester with CoA . The ACSF2 gene is also known by several aliases, including Medium-Chain Acyl-CoA Ligase and Mitochondrial Acyl-CoA Synthetase .

Gene and Protein Information

The ACSF2 gene is located on chromosome 17 and is involved in various metabolic pathways, including fatty acid metabolism and general metabolism . The protein encoded by this gene has ligase activity, which is essential for the activation of fatty acids by CoA to produce acyl-CoA . This process is the first step in fatty acid metabolism and is crucial for the proper functioning of cellular metabolic processes.

Function and Mechanism

ACSF2 enables medium-chain fatty acid-CoA ligase activity, which is predicted to be involved in the fatty acid metabolic process . The enzyme has a preference for medium-chain substrates and plays a significant role in adipocyte differentiation . This activity is essential for the conversion of fatty acids into their respective acyl-CoA derivatives, which are then utilized in various metabolic pathways.

Role in Health and Disease

The ACSF2 gene has been implicated in several health conditions due to its role in fatty acid metabolism. Dysregulation of this gene can lead to metabolic disorders and has been associated with conditions such as obesity, diabetes, and cardiovascular diseases . Additionally, ACSF2 is involved in the metabolic reprogramming of cancer cells, making it a potential target for cancer therapy .

Mouse Anti Human ACSF2 Antibody

The mouse anti-human ACSF2 antibody is used in various research applications to study the expression and function of the ACSF2 protein in human tissues. This antibody is reactive with human, mouse, and rat samples and is utilized in techniques such as Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), and Enzyme-Linked Immunosorbent Assay (ELISA) . The use of this antibody helps in understanding the role of ACSF2 in different biological processes and disease states.

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