ACP6 Human

Acid Phosphatase-6 (ACP6), also known as lysophosphatidic acid phosphatase type 6, is an enzyme that belongs to the histidine acid phosphatase family . It is a mitochondrial lipid phosphate phosphatase that plays a crucial role in regulating lipid metabolism . ACP6 is encoded by the ACP6 gene in humans and is involved in various biological processes, including lipid signaling and metabolism.

ACP6 is a protein that consists of 428 amino acids and has a molecular weight of approximately 45,313 Da . The enzyme’s structure includes a catalytic domain that is responsible for its phosphatase activity. ACP6 catalyzes the hydrolysis of lysophosphatidic acid (LPA) to monoacylglycerol, which is an important step in lipid metabolism .

Recent studies have highlighted the clinical significance of ACP6 in various diseases, particularly in hepatocellular carcinoma (HCC). ACP6 has been identified as a potential biomarker for HCC, with its overexpression being correlated with clinical progression and worse overall survival of HCC patients . Additionally, ACP6 expression is linked to immune cell interactions, including B cells, CD8+ T cells, and naive CD4+ T cells .

Recombinant ACP6 can be produced using various expression systems, including bacterial, yeast, baculovirus-insect, and mammalian expression systems . The production process typically involves gene synthesis, vector construction, protein expression, and purification. High-efficiency expression vectors and proprietary medium formulations are used to achieve high cell density culturing and efficient protein production .

ACP6 catalyzes the hydrolysis of lysophosphatidic acid (LPA) to monoacylglycerol, a reaction that is essential for lipid metabolism . This enzymatic activity is crucial for maintaining lipid homeostasis and regulating lipid signaling pathways. The enzyme’s activity can be analyzed using various biochemical assays that measure the release of phosphate from LPA substrates.