Introduction
ACOT11 is a protein that functions as an acyl-CoA thioesterase, exhibiting activity towards medium-chain (C12) and long-chain (C18) fatty acyl-CoA substrates. Studies have shown that the expression of a similar protein in murine brown adipose tissue is influenced by temperature, being upregulated by cold exposure and downregulated by warmth. Furthermore, research has linked the expression levels of mouse ACOT11 to obesity, with higher expression observed in obesity-resistant mice compared to their obesity-prone counterparts.
Formulation
The antibody is supplied as a 1 mg/ml solution in a buffer consisting of PBS at pH 7.4, 0.02% sodium azide, and 10% glycerol.
Storage Procedures
For short-term storage of up to 1 month, the antibody can be stored at 4°C. For extended storage, it is recommended to store at -20°C to ensure optimal preservation. To maintain the integrity of the antibody, it is crucial to avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The antibody exhibits a shelf life of 12 months when stored at -20°C and remains stable for 1 month when stored at 4°C.
Applications
The ACOT11 antibody has undergone rigorous testing in various applications, including ELISA, Western blot, and immunohistochemistry analysis, to confirm its specificity and reactivity. However, it's important to note that optimal working dilutions may vary depending on the specific application and experimental conditions. Therefore, it is highly recommended to perform titration experiments to determine the optimal working dilution for your particular assay. As a general guideline, the recommended dilution range for Western blot analysis is 1:500 to 1:2,000, while for immunohistochemistry analysis, a dilution range of 1:50 to 1:100 is suggested. For Western blot analysis, a starting dilution of 1:1,000 is recommended, and for immunohistochemistry, a starting dilution of 1:100 is advised.
Synonyms
Acyl-coenzyme A thioesterase 11, Acyl-CoA thioesterase 11, Acyl-CoA thioester hydrolase 11, Brown fat-inducible thioesterase, BFIT, Adipose-associated thioesterase, ACOT11, BFIT, KIAA0707, THEA, BFIT1, BFIT2, THEM1, STARD14.
Purification Method
ACOT11 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Immunogen
Anti-human ACOT11 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human ACOT11 amino acids 19-250 purified from E. coli.
Ig Subclass
Mouse IgG2b heavy chain and κ light chain.