ACOT11 Antibody

Acyl-Coenzyme A Thioesterase 11, Mouse Anti Human
Cat. No.
BT12424
Source
Synonyms
Acyl-coenzyme A thioesterase 11, Acyl-CoA thioesterase 11, Acyl-CoA thioester hydrolase 11, Brown fat-inducible thioesterase, BFIT, Adipose-associated thioesterase, ACOT11, BFIT, KIAA0707, THEA, BFIT1, BFIT2, THEM1, STARD14.
Appearance
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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In Stock

Description

Product Specs

Introduction
ACOT11 is a protein that functions as an acyl-CoA thioesterase, exhibiting activity towards medium-chain (C12) and long-chain (C18) fatty acyl-CoA substrates. Studies have shown that the expression of a similar protein in murine brown adipose tissue is influenced by temperature, being upregulated by cold exposure and downregulated by warmth. Furthermore, research has linked the expression levels of mouse ACOT11 to obesity, with higher expression observed in obesity-resistant mice compared to their obesity-prone counterparts.
Formulation
The antibody is supplied as a 1 mg/ml solution in a buffer consisting of PBS at pH 7.4, 0.02% sodium azide, and 10% glycerol.
Storage Procedures
For short-term storage of up to 1 month, the antibody can be stored at 4°C. For extended storage, it is recommended to store at -20°C to ensure optimal preservation. To maintain the integrity of the antibody, it is crucial to avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The antibody exhibits a shelf life of 12 months when stored at -20°C and remains stable for 1 month when stored at 4°C.
Applications
The ACOT11 antibody has undergone rigorous testing in various applications, including ELISA, Western blot, and immunohistochemistry analysis, to confirm its specificity and reactivity. However, it's important to note that optimal working dilutions may vary depending on the specific application and experimental conditions. Therefore, it is highly recommended to perform titration experiments to determine the optimal working dilution for your particular assay. As a general guideline, the recommended dilution range for Western blot analysis is 1:500 to 1:2,000, while for immunohistochemistry analysis, a dilution range of 1:50 to 1:100 is suggested. For Western blot analysis, a starting dilution of 1:1,000 is recommended, and for immunohistochemistry, a starting dilution of 1:100 is advised.
Synonyms
Acyl-coenzyme A thioesterase 11, Acyl-CoA thioesterase 11, Acyl-CoA thioester hydrolase 11, Brown fat-inducible thioesterase, BFIT, Adipose-associated thioesterase, ACOT11, BFIT, KIAA0707, THEA, BFIT1, BFIT2, THEM1, STARD14.
Purification Method
ACOT11 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PJ4B2AT.
Immunogen
Anti-human ACOT11 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human ACOT11 amino acids 19-250 purified from E. coli.
Ig Subclass
Mouse IgG2b heavy chain and κ light chain.

Product Science Overview

Mouse Anti-Human Antibodies

Mouse anti-human antibodies are secondary antibodies generated by immunizing mice with human immunoglobulins . These antibodies are affinity-purified and have well-characterized specificity for human immunoglobulins . They are commonly used in various applications, including detection, sorting, and purification of human targets .

Applications and Benefits

Mouse anti-human antibodies offer increased versatility, enabling the use of multiple detection systems such as horseradish peroxidase (HRP), alkaline phosphatase (AP), and fluorescence . They also provide greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody . These antibodies are widely used in techniques such as enzyme-linked immunosorbent assay (ELISA), Western blotting, flow cytometry, and immunohistochemistry .

Considerations

One important consideration when using mouse anti-human antibodies is the potential for the human anti-mouse antibody (HAMA) response . This response can range from mild allergic reactions to more severe and life-threatening responses, such as kidney failure . Therefore, it is essential to monitor and manage this response when using mouse anti-human antibodies in clinical and research settings .

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