ACAT2 Antibody
Description
Product Specs
The antibody is provided at a concentration of 1mg/ml in a buffer solution containing PBS at a pH of 7.4, 10% Glycerol, and 0.02% Sodium Azide.
Product Science Overview
Mouse anti-human antibodies are secondary antibodies generated by immunizing mice with human immunoglobulins . These antibodies are widely used in research and diagnostic applications due to their specificity and versatility .
Production and Purification
Mouse anti-human antibodies are produced by injecting mice with human immunoglobulins, which stimulates the mouse immune system to produce antibodies against the human proteins . These antibodies are then harvested from the mouse serum and purified using techniques such as immunoaffinity chromatography .
Applications
Mouse anti-human antibodies are used in various applications, including:
- Detection: They are used in assays such as ELISA, Western blotting, and immunohistochemistry to detect human proteins .
- Sorting and purification: These antibodies can be used to sort and purify human cells or proteins from a mixture .
- Signal amplification: Secondary antibodies can bind to multiple primary antibodies, amplifying the signal and increasing sensitivity in detection assays .
Considerations
One important consideration when using mouse anti-human antibodies is the potential for the human anti-mouse antibody (HAMA) response . This response occurs when the human immune system recognizes the mouse antibodies as foreign and mounts an immune response against them . The HAMA response can range from mild allergic reactions to severe complications such as kidney failure .
