ZWINT Human

ZW10 Interacting Kinetochore Protein Human Recombinant

Cat. No.

BT2221

Source

Escherichia Coli.

Synonyms

ZW10 Interacting Kinetochore Protein, ZWINT, ZW10 Interactor, HZwint-1, KNTC2AP, ZWINT1, Human ZW10 Interacting Protein-1, ZW10 Interactor Kinetochore Protein zwint-1, Zwint-1, ZW10-Interacting Protein 1.

Appearance

Sterile Filtered colorless solution.

Purity

Greater than 85.0% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

ZWINT Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 253 amino acids (1-230) and having a molecular mass of 27.9 kDa (Molecular size on SDS-PAGE will appear higher).
ZWINT is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

ZW10 Interacting Kinetochore Protein (ZWINT) plays a crucial role in kinetochore function. It is a component of the MIS12 complex, which is vital for kinetochore assembly and the spindle checkpoint's activity. ZWINT resides in the cytoplasm during interphase and associates with kinetochores from late prophase to anaphase. Interacting with ZW10 (Zeste White 10), ZWINT regulates the connection between ZW10 and kinetochores. Defects in the ZWINT gene are implicated in Roberts syndrome, an autosomal recessive disorder marked by growth delays due to premature chromosome separation.

Description

This product is a recombinant human ZWINT protein produced in E. coli. It is a single, non-glycosylated polypeptide chain consisting of 253 amino acids (residues 1-230) with a molecular weight of 27.9 kDa. Note that its size on SDS-PAGE might appear larger. This ZWINT protein is fused to a 23 amino acid His-tag at its N-terminus and purified using proprietary chromatographic methods.

Physical Appearance

Clear, colorless solution that has been sterilized by filtration.

Formulation

This ZWINT protein solution has a concentration of 0.25 mg/ml and is supplied in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.15 M NaCl, 20% glycerol, and 1 mM DTT.

Stability

For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it should be kept frozen at -20°C. To ensure stability during long-term storage, adding a carrier protein (either 0.1% HSA or BSA) is recommended. Repeated freezing and thawing of the product should be avoided.

Purity

The purity of this product is greater than 85.0% as determined by SDS-PAGE analysis.

Synonyms

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMEAAETE AEAAALEVLA EVAGILEPVG LQEEAELPAK ILVEFVVDSQ KKDKLLCSQL QVADFLQNIL AQEDTAKGLD PLASEDTSRQ KAIAAKEQWK ELKATYREHV EAIKIGLTKA LTQMEEAQRK RTQLREAFEQ LQAKKQMAME KRRAVQNQWQ LQQEKHLQHL AEVSAEGKLL FPEAEAEAEN LPDDKPQQPT RPQEQSTGDT MGRDPGVSFK AVGLQPAGDV NLP.

Product Science Overview

Introduction

ZW10 Interacting Kinetochore Protein (ZWINT) is a crucial component of the mitotic spindle checkpoint, playing a significant role in chromosome segregation during cell division. This protein is essential for the proper attachment of chromosomes to the spindle microtubules, ensuring accurate chromosome alignment and segregation. ZWINT has garnered attention due to its involvement in various cellular processes and its potential implications in cancer biology.

Discovery and Structure

ZWINT was initially identified as a protein that interacts with Zeste White 10 (ZW10), a key player in the mitotic checkpoint. The protein is characterized by its ability to bind to kinetochores, which are protein structures on chromosomes that attach to spindle fibers during cell division. ZWINT is composed of several domains that facilitate its interaction with other kinetochore proteins and microtubules, making it an integral part of the kinetochore complex.

Function and Mechanism

ZWINT functions as a scaffold protein, providing a platform for the assembly of other kinetochore proteins. It plays a pivotal role in the recruitment of the RZZ (Rod-ZW10-Zwilch) complex to the kinetochores, which is essential for the activation of the spindle assembly checkpoint (SAC). The SAC ensures that chromosomes are correctly attached to the spindle microtubules before the cell proceeds to anaphase, thereby preventing chromosome missegregation and aneuploidy.

Role in Cancer

Recent studies have highlighted the upregulation of ZWINT in various types of human cancers, including breast cancer. Elevated levels of ZWINT have been associated with poor prognosis and aggressive tumor characteristics. For instance, high ZWINT expression has been linked to positive human epidermal growth factor receptor 2 (HER2) expression, triple-negative breast cancer, younger age, basal-like subtype, and higher Scarff-Bloom-Richardson grades . These findings suggest that ZWINT may serve as a potential prognostic biomarker and therapeutic target in cancer treatment.

Research and Applications

The recombinant form of ZWINT, produced through genetic engineering techniques, has been instrumental in studying its function and interactions. Human recombinant ZWINT is used in various research applications, including the investigation of its role in mitotic checkpoint signaling, chromosome segregation, and cancer progression. Additionally, recombinant ZWINT is utilized in drug discovery and development, aiming to identify compounds that can modulate its activity for therapeutic purposes.

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ZWINT Human

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