ZWILCH Human

Zwilch Kinetochore Protein Human Recombinant
Cat. No.
BT2182
Source
Escherichia Coli.
Synonyms
hZwilch, KNTC1AP, Protein zwilch homolog, ZWILCH.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ZWILCH Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 614 amino acids (1-591 a.a) and having a molecular mass of 69.6kDa.
ZWILCH is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The ZWILCH Kinetochore Protein, also known as ZWILCH, belongs to the ZWILCH family and plays a crucial role in the mitotic checkpoint. This protein prevents premature exit from mitosis and is essential for the assembly of the dynein-dynactin and MAD1-MAD2 complexes at kinetochores.
Description
Recombinant human ZWILCH, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 614 amino acids (residues 1-591) with a molecular weight of 69.6 kDa. This protein includes a 23 amino acid His-tag at the N-terminus and undergoes purification using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
This solution contains 0.25 mg/ml of ZWILCH protein in a buffer composed of 20 mM Tris-HCl (pH 8.0), 0.1 M NaCl, 20% glycerol, and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), the solution should be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freezing and thawing cycles should be avoided.
Purity
Purity levels exceed 85.0% as determined by SDS-PAGE analysis.
Synonyms
hZwilch, KNTC1AP, Protein zwilch homolog, ZWILCH.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMWERLNC AAEDFYSRLL QKFNEEKKGI RKDPFLYEAD VQVQLISKGQ PNPLKNILNE NDIVFIVEKV PLEKEETSHI EELQSEETAI SDFSTGENVG PLALPVGKAR QLIGLYTMAH NPNMTHLKIN LPVTALPPLW VRCDSSDPEG TCWLGAELIT TNNSITGIVL YVVSCKADKN YSVNLENLKN LHKKRHHLST VTSKGFAQYE LFKSSALDDT ITASQTAIAL DISWSPVDEI LQIPPLSSTA TLNIKVESGE PRGPLNHLYR ELKFLLVLAD GLRTGVTEWL EPLEAKSAVE LVQEFLNDLN KLDGFGDSTK KDTEVETLKH DTAAVDRSVK RLFKVRSDLD FAEQLWCKMS SSVISYQDLV KCFTLIIQSL QRGDIQPWLH SGSNSLLSKL IHQSYHGTMD TVSLSGTIPV QMLLEIGLDK LKKDYISFFI GQELASLNHL EYFIAPSVDI QEQVYRVQKL HHILEILVSC MPFIKSQHEL LFSLTQICIK YYKQNPLDEQ HIFQLPVRPT AVKNLYQSEK PQKWRVEIYS GQKKIKTVWQ LSDSSPIDHL NFHKPDFSEL TLNGSLEERI FFTNMVTCSQ VHFK.

Product Science Overview

Introduction

The Zwilch Kinetochore Protein, also known as ZWILCH, is a crucial component of the kinetochore complex in human cells. It plays a significant role in the proper segregation of chromosomes during cell division, ensuring that each daughter cell receives the correct number of chromosomes. This protein is encoded by the ZWILCH gene and is essential for maintaining genomic stability.

Gene and Protein Structure

The ZWILCH gene is located on chromosome 15 and encodes a protein that is part of the RZZ (ROD-ZW10-Zwilch) complex . This complex is essential for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores, which are critical for the mitotic checkpoint . The ZWILCH protein itself is composed of several domains that facilitate its interaction with other proteins in the kinetochore complex.

Function and Mechanism

ZWILCH is an essential component of the mitotic checkpoint, a safety mechanism that prevents cells from prematurely exiting mitosis . It ensures that chromosomes are correctly attached to the spindle apparatus before the cell proceeds to anaphase. The protein’s function is closely related to its association with the RZZ complex, which is necessary for the proper localization of other kinetochore proteins .

Role in Disease

Mutations or dysregulation of the ZWILCH gene can lead to chromosomal instability, which is a hallmark of many cancers. For instance, defects in the ZWILCH protein have been associated with Buschke-Ollendorff Syndrome, a rare genetic disorder . Research is ongoing to understand the full spectrum of diseases associated with ZWILCH dysfunction.

Research and Applications

The study of ZWILCH and its role in the kinetochore complex has significant implications for cancer research and the development of therapeutic strategies. By understanding how ZWILCH functions in normal and diseased cells, scientists can develop targeted therapies to correct or mitigate the effects of its dysfunction.

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