ZNF514 Human
Zinc Finger Protein 514 Human Recombinant
Cat. No.
BT28505
Source
Escherichia Coli.
Synonyms
Zinc
finger protein 514, ZNF514.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
ZNF514 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 423 amino acids (1-400 a.a) and having a molecular mass of 48.3kDa.
ZNF514 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Zinc finger protein 514 (ZNF514) belongs to a specific protein family and possesses structural features typical of this family. It contains zinc finger domains and a KRAB domain. ZNF514 might be involved in controlling gene expression.
Description
This product consists of the human ZNF514 protein produced in E. coli bacteria. It is a single chain of amino acids, without any sugar modifications, and has a specific molecular weight. The protein includes a His-tag for purification purposes and is purified using specialized techniques.
Physical Appearance
A clear solution that has been sterilized by filtration.
Formulation
The ZNF514 protein is provided in a solution with a concentration of 1mg/ml. This solution contains a buffer to maintain pH, urea, and glycerol.
Stability
For short-term storage (up to 2-4 weeks), keep at 4°C. For longer periods, freeze at -20°C. Adding a carrier protein is recommended for long-term storage. Avoid repeated freezing and thawing.
Purity
The purity of the ZNF514 protein is greater than 85%, as determined by SDS-PAGE analysis.
Synonyms
Zinc
finger protein 514, ZNF514.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMTFEDVA VEFSQWEWGQ LNPAQKDLYR EVMLENFRNL AILGLLVSKP
YVICQLEEGG EPFMVEREIS TGAHSDWKRR SKSKESMPSW GISKEELFQV VSVEKHIQDV LQFSKLKAAC
GCDGQLEMQQ IKQERHLKQM STIHKSATTL SRDYKWNGFG RSLGLRSVLV NQHSILMGEG SYKCDTEFRQ TLGGNNSQRT HPEKKSCKCN
ECGKSFHFQS ELRRHQRCHT GEKPYECSDC GRAFGHISSL IKHQRTHTGE KPYECSECGR AFSQSSSLVL
HYRFHTGEKP YKCNECGRAF GHTSSLIKHQ RTHTGEKPYE CRECGRTFSQ SSSLIVHYRF HTGEKPYKCN KCGRAFSQSS SLTQHYRFHT GEKPYKCNEC GRAFAHTASL
IKHQRSHAGK KTL
Product Science Overview
Structure and Domains
ZNF514 contains:
- Seven C2H2-type zinc fingers: These are classical zinc finger motifs that typically bind to DNA and are involved in transcriptional regulation.
- One KRAB domain: The Krueppel-associated box (KRAB) domain is a transcriptional repression domain found in many zinc finger proteins. It is involved in mediating protein-protein interactions and can recruit other proteins to form complexes that regulate gene expression .
Function
ZNF514 is believed to be involved in transcriptional regulation. Transcription factors like ZNF514 can bind to specific DNA sequences and control the rate of transcription of genetic information from DNA to messenger RNA. This regulation is essential for various cellular processes, including cell growth, differentiation, and response to environmental signals .
Recombinant Human ZNF514
Recombinant human ZNF514 is produced using Escherichia coli (E. coli) as the expression host. The recombinant protein is typically fused to a His-tag at the N-terminus, which facilitates its purification. The protein is expressed as a single, non-glycosylated polypeptide chain containing 423 amino acids and has a molecular mass of approximately 48.3 kDa .
Applications
Recombinant ZNF514 is used in various research applications, including:
- Studying gene regulation: Understanding how ZNF514 interacts with DNA and other proteins to regulate gene expression.
- Protein-protein interactions: Investigating the interactions between ZNF514 and other proteins, which can provide insights into its role in cellular processes.
- Functional assays: Assessing the functional activity of ZNF514 in different biological contexts .
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