ZFAND5 Human
Zinc Finger, AN1-Type Domain 5 Human Recombinant
Cat. No.
BT28118
Source
Escherichia Coli.
Synonyms
Zinc Finger, AN1-Type Domain 5, Zinc Finger Protein 216, ZNF216,
Zinc Finger A20 Domain-Containing Protein 2, Zinc Finger, A20 Domain Containing
2, ZA20D2, AN1-Type Zinc Finger Protein 5, ZFAND5A, AN1-type zinc finger
protein 5, Zinc finger A20 domain-containing protein 2, Zinc finger protein 216.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
ZFAND5 Human Recombinant produced in E.Coli is a single, non-glycosylated
polypeptide chain containing 236 amino acids (1-213 a.a) and having a molecular
mass of 25.5kDa (Molecular size on SDS-PAGE will appear higher).
ZFAND5 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Product Specs
Introduction
Zinc Finger, AN1-Type Domain 5, also known as ZFAND5, is a protein involved in protein degradation via the ubiquitin-proteasome system. ZFAND5 functions by binding to ubiquitinated proteins and delivering them to the proteasome for degradation. This process is important for muscle atrophy. Additionally, ZFAND5 plays a role in regulating NF-kappa-B activation and apoptosis. It can inhibit NF-kappa-B activation induced by TNF, IL-1, and TLR4.
Description
Recombinant human ZFAND5 protein was expressed in E. coli and purified to a single, non-glycosylated polypeptide chain. This protein consists of 236 amino acids (with amino acids 1-213 present) and has a molecular weight of 25.5 kDa. Note: The molecular size observed on SDS-PAGE may be higher.
This ZFAND5 protein is fused to a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
This ZFAND5 protein is fused to a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The ZFAND5 protein solution is provided at a concentration of 0.5 mg/ml and contains 20 mM phosphate buffered saline (pH 8.0), 30% glycerol, and 1 mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the protein solution should be stored at 4°C. For long-term storage, the protein solution should be stored at -20°C.
It is recommended to add a carrier protein (such as 0.1% HSA or BSA) for long-term storage.
Repeated freeze-thaw cycles should be avoided.
It is recommended to add a carrier protein (such as 0.1% HSA or BSA) for long-term storage.
Repeated freeze-thaw cycles should be avoided.
Purity
The purity of the protein is greater than 85.0% as determined by SDS-PAGE analysis.
Synonyms
Zinc Finger, AN1-Type Domain 5, Zinc Finger Protein 216, ZNF216,
Zinc Finger A20 Domain-Containing Protein 2, Zinc Finger, A20 Domain Containing
2, ZA20D2, AN1-Type Zinc Finger Protein 5, ZFAND5A, AN1-type zinc finger
protein 5, Zinc finger A20 domain-containing protein 2, Zinc finger protein 216.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAQETNQ TPGPMLCSTG CGFYGNPRTN GMCSVCYKEH LQRQQNSGRM
SPMGTASGSN SPTSDSASVQ RADTSLNNCE GAAGSTSEKS RNVPVAALPV TQQMTEMSIS REDKITTPKT
EVSEPVVTQP SPSVSQPSTS QSEEKAPELP KPKKNRCFMC RKKVGLTGFD CRCGNLFCGL HRYSDKHNCP
YDYKAEAAAK IRKENPVVVA EKIQRI.
Product Science Overview
Introduction
Zinc Finger, AN1-Type Domain 5 (ZFAND5), also known as Zinc Finger Protein 216 (ZNF216), is a protein encoded by the ZFAND5 gene in humans. This protein is characterized by the presence of zinc finger motifs, which are small protein structural motifs that can coordinate one or more zinc ions to help stabilize their folds. ZFAND5 is involved in various biological processes, including protein degradation, regulation of NF-kappa-B activation, and apoptosis.
Structure
ZFAND5 is a single-chain, circular protein with a molecular weight of approximately 28 kDa . It contains several key structural features:
- Zinc Finger Structure: This motif includes a zinc ion that helps stabilize the protein structure.
- AN1-Type Domain: A conserved amino acid sequence involved in the modification of cysteine residues in proteins.
- Conserved Cysteine Sites: These sites are crucial for the specific recognition of drug targets.
- Disulfide Bonds: These bonds contribute to the stability of the protein.
- Glycosylation Modifications: These modifications are significant for the protein’s function .
Biological Functions
ZFAND5 plays a critical role in several biological processes:
- Protein Degradation: ZFAND5 is involved in the ubiquitin-proteasome system, anchoring ubiquitinated proteins to the proteasome for degradation. This process is essential during muscle atrophy .
- Regulation of NF-kappa-B Activation: ZFAND5 inhibits NF-kappa-B activation triggered by various stimuli, including tumor necrosis factor (TNF), IL-1, and TLR4. Overexpression of ZFAND5 sensitizes cells to TNF-induced apoptosis .
- Apoptosis: ZFAND5 plays a role in the regulation of apoptosis, contributing to cell death processes .
Clinical Significance
ZFAND5 has been identified as a potential biomarker and drug target in various diseases:
- Cancer: ZFAND5 is a key drug target in many tumor cells. Its expression levels are significantly correlated with tumor invasion, growth rate, and cell cycle .
- Neurodegenerative Diseases: ZFAND5 is implicated in neurodegenerative diseases such as Alzheimer’s and Parkinson’s disease. It may serve as a new target for therapeutic interventions .
- Immune Diseases: ZFAND5 affects immune cell functions and is involved in the pathogenesis of immune diseases .
Research and Applications
ZFAND5 is widely studied for its potential applications in drug development and disease treatment:
- Drug Development: As a biomarker, ZFAND5 provides new insights for drug development. By detecting ZFAND5 expression levels and binding drugs, researchers can identify specific drug targets .
- Biomarker Research: ZFAND5 is used in biomarker research for disease diagnosis, prognosis, and treatment monitoring. For example, it can be a biomarker for Parkinson’s disease .
- Target Research: ZFAND5 is a valuable target for drug research. By studying the interaction between drugs and ZFAND5, researchers can gain important insights into drug mechanisms .
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
