YY1 Human
YY1 Transcription Factor Human Recombinant
Cat. No.
BT1780
Source
Escherichia Coli.
Synonyms
YY1 transcription factor, YY1, DELTA, INO80S, NF-E1, UCRBP, YIN-YANG-1, Transcriptional repressor protein YY1, Delta transcription factor, Yin and yang 1, INO80 complex subunit S, YY-1.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
YY1 Human Recombinant produced in E. coli is a single polypeptide chain containing 437 amino acids (1-414) and having a molecular mass of 47.1kDa.
YY1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
YY1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
The YY1 Transcription Factor, also referred to as YY1, belongs to the GLI-Kruppel family of zinc finger proteins. YY1 can both positively and negatively regulate a variety of cellular and viral genes by interacting with specific DNA regions that overlap with the transcription start site. YY1 exerts its regulatory effects by recruiting histone deacetylases and histone acetyltransferases to promoters, thereby influencing their activation or repression.
Description
Recombinant Human YY1, produced in E. coli, is a single polypeptide chain with a molecular weight of 47.1kDa. It comprises 437 amino acids, spanning from position 1 to 414. The protein is engineered with a 23 amino acid His-tag fused to its N-terminus. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
The product appears as a clear solution that has undergone sterile filtration.
Formulation
The YY1 solution is provided at a concentration of 0.25mg/ml and is formulated in a buffer consisting of PBS (phosphate-buffered saline), 20% glycerol, and 1mM DTT (dithiothreitol).
Stability
For short-term storage (2-4 weeks), the product should be stored at 4°C. For extended storage, it is recommended to store the product frozen at -20°C. To ensure optimal stability during long-term storage, adding a carrier protein like HSA (human serum albumin) or BSA (bovine serum albumin) at a concentration of 0.1% is advisable. It is important to avoid repeated cycles of freezing and thawing.
Purity
Analysis by SDS-PAGE indicates a purity level exceeding 80.0%.
Synonyms
YY1 transcription factor, YY1, DELTA, INO80S, NF-E1, UCRBP, YIN-YANG-1, Transcriptional repressor protein YY1, Delta transcription factor, Yin and yang 1, INO80 complex subunit S, YY-1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMASGDTL YIATDGSEMP AEIVELHEIE VETIPVETIE TTVVGEEEEE DDDDEDGGGG DHGGGGGHGH AGHHHHHHHH HHHPPMIALQ PLVTDDPTQV HHHQEVILVQ TREEVVGGDD SDGLRAEDGF EDQILIPVPA PAGGDDDYIE QTLVTVAAAG KSGGGGSSSS GGGRVKKGGG KKSGKKSYLS GGAGAAGGGG ADPGNKKWEQ KQVQIKTLEG EFSVTMWSSD EKKDIDHETV VEEQIIGENS PPDYSEYMTG KKLPPGGIPG IDLSDPKQLA EFARMKPRKI KEDDAPRTIA CPHKGCTKMF RDNSAMRKHL HTHGPRVHVC AECGKAFVES SKLKRHQLVH TGEKPFQCTF EGCGKRFSLD FNLRTHVRIH TGDRPYVCPF DGCNKKFAQS TNLKSHILTH AKAKNNQ.
Product Science Overview
Introduction
Structure and Function
YY1 is characterized by its ability to act as both a transcriptional activator and repressor, depending on the context. This dual functionality is reflected in its name, “Yin Yang,” symbolizing its capacity to balance opposing forces . The protein contains four C2H2 zinc fingers in its C-terminal domain, which enable it to bind to specific DNA sequences and regulate gene expression .
Mechanisms of Action
YY1 regulates gene expression through several mechanisms:
- Transcriptional Activation and Repression: YY1 can bind to promoter regions of genes and either activate or repress their transcription. This is achieved by recruiting various cofactors and chromatin modifiers, such as histone deacetylases (HDACs) and histone acetyltransferases (HATs), to the promoter .
- Chromatin Looping: YY1 promotes the formation of enhancer-promoter chromatin loops by forming dimers and facilitating DNA interactions. This looping is essential for the regulation of gene expression over long distances .
- Epigenetic Modifications: YY1 is involved in recruiting proteins required for epigenetic modifications, including acetylation, deacetylation, methylation, ubiquitination, and sumoylation .
Biological Significance
YY1 plays a fundamental role in various biological processes, including:
- Embryogenesis: YY1 is essential for the proper development of embryos, influencing processes such as differentiation and proliferation .
- Neurogenesis: YY1 is crucial for the development and maintenance of the central nervous system, where it regulates neurogenesis and homeostasis .
- Disease Association: Dysregulation of YY1 has been linked to several diseases, including Gabriele-DeVries syndrome (GADEVS), an autosomal dominant neurodevelopmental disorder characterized by intellectual disability and other congenital malformations .
Recombinant Production
The human recombinant YY1 transcription factor is produced using recombinant DNA technology. This involves cloning the YY1 gene into an expression vector, which is then introduced into a host cell (such as E. coli or mammalian cells) for protein production. The recombinant protein is subsequently purified using various chromatographic techniques to ensure high purity and functionality .
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