YWHAE Antibody

Tyr-3/Trp- 5 Monooxygenase Activation Protein Epsilon, Mouse Anti Human
Cat. No.
BT27723
Source
Synonyms
YWHAE, MDS, MDCR, KCIP-1, 14-3-3E, 14-3-3 Epsilon, FLJ45465, Tyr-3/Trp- 5 Monooxygenase Activation Protein Epsilon.
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Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic, and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ, and η, that have been identified in mammals. The 14-3-3 epsilon, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with CDC25 phosphatases, RAF1, and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer.
Formulation
1 mg/ml containing PBS, pH 7.4, 10% Glycerol, and 0.02% Sodium Azide.
Storage Procedures
For periods up to 1 month, store at 4 °C; for longer periods, store at -20 °C. Prevent freeze-thaw cycles.
Stability / Shelf Life
12 months at -20 °C. 1 month at 4 °C.
Applications
YWHAE antibody has been tested by ELISA, Western blot analysis, flow cytometry, and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Synonyms
YWHAE, MDS, MDCR, KCIP-1, 14-3-3E, 14-3-3 Epsilon, FLJ45465, Tyr-3/Trp- 5 Monooxygenase Activation Protein Epsilon.
Purification Method
YWHAE antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT4F8AT.
Immunogen
Anti-human YWHAE mAb is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with recombinant human YWHAE amino acids 1-255 purified from E. coli.
Ig Subclass
Mouse IgG2b heavy chain and k light chain

Product Science Overview

Introduction

Tyr-3/Trp-5 Monooxygenase Activation Protein Epsilon, also known as YWHAE or 14-3-3 epsilon, is a member of the 14-3-3 protein family. These proteins are highly conserved and ubiquitously expressed in eukaryotic cells. They play crucial roles in various cellular processes, including signal transduction, cell cycle control, and apoptosis.

Structure and Function

The 14-3-3 proteins, including YWHAE, function as adaptor proteins that mediate protein-protein interactions. They typically form dimers and bind to phosphorylated serine/threonine residues on target proteins. This binding can alter the target protein’s function, localization, or stability, thereby influencing various cellular pathways.

Biological Significance

YWHAE is involved in several critical cellular processes:

  • Signal Transduction: It modulates the activity of various kinases and phosphatases, thereby influencing signaling pathways.
  • Cell Cycle Control: YWHAE interacts with proteins involved in cell cycle regulation, ensuring proper cell division and proliferation.
  • Apoptosis: It plays a role in the regulation of apoptosis by interacting with pro-apoptotic and anti-apoptotic proteins.
Mouse Anti Human YWHAE Antibody

The Mouse Anti Human YWHAE antibody is a monoclonal antibody developed to specifically recognize and bind to the human YWHAE protein. This antibody is used in various research applications, including:

  • Western Blotting: To detect YWHAE protein levels in different samples.
  • Immunoprecipitation: To isolate YWHAE and its interacting partners.
  • Immunofluorescence: To visualize the localization of YWHAE within cells.
Applications in Research

The Mouse Anti Human YWHAE antibody is a valuable tool in research focused on understanding the role of YWHAE in cellular processes and disease mechanisms. It is particularly useful in studies related to:

  • Cancer: Investigating the role of YWHAE in tumorigenesis and cancer progression.
  • Neurodegenerative Diseases: Exploring the involvement of YWHAE in diseases such as Alzheimer’s and Parkinson’s.
  • Developmental Biology: Studying the function of YWHAE in embryonic development and differentiation.

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THE BIOTEK
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