Y.Enterocolitica (O:9) YopB
Sf9, Baculovirus cells.
Protein YopD, pYV0054.
Sterile Filtered clear solution.
Greater than 80.0% as determined by SDS-PAGE.
Description
Recombinant Yersinia Enterocolitica (O:9) YopB produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain having a calculated molecular mass of 43kDa.
Y.Enterocolitica (O:9) YopB is expressed with a -10x His tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Yersinia enterocolitica is a bacterium that can cause gastroenteritis. Virulent strains of this bacterium produce a protein called YopB, which helps to form pores in the host cell membrane. These pores allow other Yersinia proteins, called effectors, to enter the host cell and disrupt its normal function.
This product is a recombinant form of the YopB protein from Yersinia enterocolitica serotype O:9. It is produced in insect cells and purified to a high degree. The protein is glycosylated, meaning it has sugars attached to it, and has a molecular weight of approximately 43 kDa. The protein also includes a His tag to facilitate purification.
The product is a clear, colorless, and sterile solution.
This recombinant Y.Enterocolitica (O:9) YopB protein is supplied in a buffer solution containing 20mM HEPES (pH 7.6), 250mM NaCl, and 20% glycerol.
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to store the product frozen at -20°C. Repeated freezing and thawing of the product should be avoided to maintain its quality.
The purity of this product is greater than 80%, as determined by SDS-PAGE analysis.
Protein YopD, pYV0054.
Sf9, Baculovirus cells.
2. Immunodot test with positive/negative samples.
Product Science Overview
Yersinia enterocolitica is a Gram-negative bacterium that is known to cause yersiniosis, a zoonotic disease that affects both humans and animals. This pathogen is of significant epidemiological importance due to its ability to cause gastrointestinal infections and other severe complications . Among the various serotypes of Yersinia enterocolitica, the O:9 serotype is particularly noteworthy.
Yersinia enterocolitica produces a range of virulence factors, including a set of proteins known as Yersinia outer proteins (Yops). These proteins play a crucial role in the bacterium’s ability to evade the host immune system and establish infection . One of the key Yops is YopB, which is involved in the translocation of other Yops into host cells.
The YopB protein is an essential component of the type III secretion system (T3SS) of Yersinia enterocolitica. This system is a needle-like structure that injects effector proteins directly into the host cells, disrupting their normal functions and aiding in bacterial survival and replication . YopB, along with YopD, forms a pore in the host cell membrane through which other effector proteins are translocated.
The recombinant form of YopB is produced using genetic engineering techniques. In this process, the gene encoding YopB is inserted into a suitable expression system, such as a baculovirus-infected insect cell line (e.g., Spodoptera frugiperda Sf9 cells). This allows for the production of large quantities of the protein for research and diagnostic purposes .
Recombinant YopB is used in various immunological assays to study the immune response to Yersinia enterocolitica infections. It is also employed in the development of diagnostic tests and vaccines. The protein’s ability to bind to human antibodies (IgG, IgM, and IgA) makes it a valuable tool in these applications .
