Yellow Fever Virus
Escherichia Coli.
Sterile Filtered clear solution.
Protein is >95% pure as determined by 10% PAGE (coomassie staining).
Description
Recombinant Yellow Fever Virus produced in E. coli migrates at 12kDa.
Recombinant Yellow Fever Virus is fused to a 6xHis tag at its C-terminus and purified by proprietary chromatographic technique.
Product Specs
Escherichia Coli.
Product Science Overview
Yellow fever is a viral disease caused by the yellow fever virus, a member of the Flavivirus genus within the Flaviviridae family. It is transmitted to humans through the bites of infected mosquitoes, primarily Aedes and Haemagogus species . The disease can cause severe symptoms, including fever, jaundice, and hemorrhage, and can be fatal in some cases .
The yellow fever virus is an enveloped, positive-stranded RNA virus with a genome approximately 11 kilobases in length . The genome encodes a single polypeptide that is proteolytically processed into functional proteins necessary for viral replication . The virus has been a significant public health concern, particularly in tropical regions of Africa and South America, where it is endemic .
The live-attenuated yellow fever 17D vaccine is one of the most effective viral vaccines available today . Developed in the 1930s, it has been used for over 70 years to prevent yellow fever. The vaccine is prepared from infected chicken embryos and induces long-lasting immunity in over 95% of vaccinees within 10 days . The safety record of the 17D vaccine is outstanding, with serious adverse reactions being extremely rare .
Recombinant yellow fever viruses have been genetically engineered to carry and express foreign antigenic sequences. These recombinant viruses are being explored as potential therapeutic vaccines for various diseases, including cancer . For example, recombinant yellow fever viruses expressing a cytotoxic T-lymphocyte epitope derived from chicken ovalbumin have been shown to induce protective immunity against malignant melanoma in mice .
The construction of recombinant yellow fever viruses involves inserting foreign genes into the viral genome. One approach described involves the insertion of a green fluorescent protein variant (EGFP) into the yellow fever 17D virus . This methodology takes into account the presence of functional motifs and amino acid sequence conservation flanking the E and NS1 intergenic region to ensure the correct processing of the viral polyprotein precursor . The recombinant virus is then grown in cell cultures, such as Vero cells, and its stability and immunogenicity are assessed .
Recombinant yellow fever viruses have several potential applications:
- Vaccine Development: They can be used to develop new live-attenuated vaccines for various diseases by expressing foreign antigens.
- Cancer Therapy: Recombinant viruses have shown promise as therapeutic vaccines for treating solid tumors and pulmonary metastases in experimental models .
- Research: Insertion of foreign genes into the flavivirus genome allows for in vivo studies on flavivirus cell and tissue tropism, as well as cellular processes related to flavivirus infection .
