YEATS4 Human

YEATS Domain Containing 4 Human Recombinant
Cat. No.
BT1584
Source
E.coli.
Synonyms
YEATS domain-containing protein 4, Glioma-amplified sequence 41, Gas41, NuMA-binding protein 1, NuBI-1, NuBI1, YEATS4, GAS41, YAF9, 4930573H17Rik, B230215M10Rik.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

YEATS4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 250 amino acids (1-227 a.a.) and having a molecular mass of 28.9kDa.
YEATS4 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
YEATS Domain Containing 4 (YEATS4) shares significant sequence similarity with human MLLT1 and yeast and human MLLT3 proteins. Both MLLT1 and MLLT3 are classified as transcription factors, suggesting that YEATS4 might also function as a transcription factor. It is proposed that YEATS4 is involved in RNA transcription. Additionally, amplification of the YEATS gene has been observed in tumor cells.
Description
Recombinant human YEATS4 protein was expressed in E. coli and purified to a single, non-glycosylated polypeptide chain. The protein consists of 250 amino acids (with amino acids 1-227 present) and has a molecular weight of 28.9 kDa. For purification purposes, a 23 amino acid His-tag is present at the N-terminus. The protein was purified using proprietary chromatographic methods.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The YEATS4 protein is provided in a solution at a concentration of 0.5 mg/ml. The solution contains 20 mM Tris-HCl buffer (pH 8.0), 30% glycerol, 2 mM DTT, 0.2 M NaCl, and 0.1 mM PMSF.
Stability
For short-term storage (2-4 weeks), the protein solution should be stored at 4°C. For longer storage, it is recommended to store the solution frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is greater than 90% as assessed by SDS-PAGE analysis.
Synonyms
YEATS domain-containing protein 4, Glioma-amplified sequence 41, Gas41, NuMA-binding protein 1, NuBI-1, NuBI1, YEATS4, GAS41, YAF9, 4930573H17Rik, B230215M10Rik.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMFKRMAE FGPDSGGRVK GVTIVKPIVY GNVARYFGKK REEDGHTHQW TVYVKPYRNE DMSAYVKKIQ FKLHESYGNP LRVVTKPPYE ITETGWGEFE IIIKIFFIDP NERPVTLYHL LKLFQSDTNA MLGKKTVVSE FYDEMIFQDP TAMMQQLLTT SRQLTLGAYK HETEFAELEV KTREKLEAAK KKTSFEIAEL KERLKASRET INCLKNEIRK LEEDDQAKDI.

Product Science Overview

Discovery and Structure

YEATS4 was first identified and isolated from glioblastoma in 1997 . The YEATS domain is a highly conserved region found in several proteins involved in chromatin modification and transcriptional regulation . YEATS4 is a nuclear protein that plays a crucial role in chromatin remodeling and epigenetic regulation .

Function and Mechanism

YEATS4 is a component of the NuA4 histone acetyltransferase (HAT) complex, which is involved in the transcriptional activation of select genes by acetylating nucleosomal histones H4 and H2A . Specifically, YEATS4 recognizes and binds to acylated histone H3, with a preference for histone H3 diacetylated at lysine 18 and lysine 27 (H3K18ac and H3K27ac) or histone H3 diacetylated at lysine 14 and lysine 27 (H3K14ac and H3K27ac) . This binding facilitates the deposition of histone variant H2AZ1/H2A.Z into specific chromatin regions, promoting the transcription of actively transcribed genes .

Role in Cancer

YEATS4 has been implicated in the development and progression of various cancers. It is amplified and overexpressed in several malignancies, including astrocytomas, uterine fibroids, liposarcoma, breast cancer, liver cancer, pancreatic cancer, gastric cancer, non-small cell lung cancer, colorectal cancer, and ovarian cancer . The upregulation of YEATS4 promotes DNA damage repair and prevents cell death, while its downregulation inhibits DNA replication and induces apoptosis . Aberrant activation of YEATS4 is associated with changes in drug resistance, epithelial-mesenchymal transition, and the migration and invasion capacity of tumor cells .

Potential as a Therapeutic Target

Given its role in cancer progression, YEATS4 has emerged as a potential target for cancer therapy. Specific inhibition of YEATS4 expression or activity may be an effective strategy for inhibiting the proliferation, motility, differentiation, and survival of tumor cells . Research is ongoing to develop small-molecule inhibitors that target YEATS4 and to further understand its biological functions, metabolism, and regulatory mechanisms in various cancers .

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