YBX1 Human

Recombinant Human Y Box Binding Protein 1
Cat. No.
BT1520
Source
Escherichia Coli.
Synonyms
Y Box Binding Protein, NSEP1,DBPB, YB1, CCAAT-Binding Transcription Factor I Subunit A, Nuclease Sensitive Element Binding Protein, Enhancer Factor I Subunit A, Y-Box Transcription Factor, Y-Box-Binding Protein, DNA-Binding P, BP-8, CSDA2, CSDB, DBPB, MDR-NF1, NSEP-1, NSEP1, YB-1, YB1, EFI-A, YB-1, Major Histocompatibility Complex Class II Y Box-Binding Protein I.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

YBX1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 347 amino acids (1-324a.a) and having a molecular mass of 38.3kDa (molecular size on SDS-PAGE will appear higher). YBX1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
As a member of the CRD-mediated complex, Y Box Binding Protein 1 (YBX1) plays a crucial role in stabilizing MYC mRNA. It is involved in various cellular processes, including the translation of mammalian selenoproteins and the regulation of pre-mRNA alternative splicing. Due to its involvement in the replication of adenovirus type 5, a widely used vector in gene therapy, YBX1 has emerged as a potential drug target for cancer treatment.
Description
Recombinantly produced in E. coli, YBX1 Human Recombinant is a single, non-glycosylated polypeptide chain comprising 347 amino acids (1-324a.a). With a molecular mass of 38.3kDa, it appears larger on SDS-PAGE due to the presence of a 23 amino acid His-tag fused to its N-terminus. The protein is purified using proprietary chromatographic techniques.
Physical Appearance
A clear solution that has undergone sterile filtration.
Formulation
The YBX1 protein solution is provided at a concentration of 0.5mg/ml and is prepared in Phosphate Buffered Saline (pH7.4) with 20% glycerol.
Stability
For optimal storage, the solution should be kept at 4°C if it will be used within 2-4 weeks. For extended storage, it is recommended to freeze the solution at -20°C. The addition of a carrier protein, such as 0.1% HSA or BSA, is advisable for long-term storage. It is important to avoid repeated freezing and thawing of the solution.
Purity
SDS-PAGE analysis indicates that the purity of the protein is greater than 85.0%.
Synonyms
Y Box Binding Protein, NSEP1,DBPB, YB1, CCAAT-Binding Transcription Factor I Subunit A, Nuclease Sensitive Element Binding Protein, Enhancer Factor I Subunit A, Y-Box Transcription Factor, Y-Box-Binding Protein, DNA-Binding P, BP-8, CSDA2, CSDB, DBPB, MDR-NF1, NSEP-1, NSEP1, YB-1, YB1, EFI-A, YB-1, Major Histocompatibility Complex Class II Y Box-Binding Protein I.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSSEAET QQPPAAPPAA PALSAADTKP GTTGSGAGSG GPGGLTSAAP AGGDKKVIAT KVLGTVKWFN VRNGYGFINR NDTKEDVFVH QTAIKKNNPR KYLRSVGDGE TVEFDVVEGE KGAEAANVTG PGGVPVQGSK YAADRNHYRR YPRRRGPPRN YQQNYQNSES GEKNEGSESA PEGQAQQRRP YRRRRFPPYY MRRPYGRRPQ YSNPPVQGEV MEGADNQGAG EQGRPVRQNM YRGYRPRFRR GPPRQRQPRE DGNEEDKENQ GDETQGQQPP QRRYRRNFNY RRRRPENPKP QDGKETKAAD PPAENSSAPE AEQGGAE.

Product Science Overview

Introduction

Recombinant Human Y Box Binding Protein 1 (YBX1) is a multifunctional protein that plays a crucial role in various cellular processes. It is a member of the Cold Shock Domain protein family and is known for its ability to bind both DNA and RNA. YBX1 is involved in transcription regulation, RNA stabilization, mRNA splicing, DNA repair, and translational repression .

Structure

YBX1 consists of 324 amino acids and has a molecular mass of approximately 38.3 kDa . The protein contains a Cold Shock Domain, which is responsible for its nucleic acid-binding properties. This domain allows YBX1 to interact with specific RNA and DNA sequences, thereby influencing various cellular functions .

Functions
  1. RNA Binding and Stabilization: YBX1 preferentially binds to the 5’-[CU]CUGCG-3’ RNA motif and recognizes mRNA transcripts modified by C5-methylcytosine (m5C). This binding promotes mRNA stabilization by preventing mRNA decay .
  2. Transcription Regulation: YBX1 can bind to promoters containing a Y-box (5’-CTGATTGGCCAA-3’), such as those of the MDR1 and HLA class II genes. This binding regulates the transcription of these genes .
  3. DNA Repair: YBX1 promotes the separation of DNA strands that contain mismatches or are modified by cisplatin. It also has endonucleolytic activity, introducing nicks or breaks into double-stranded DNA, suggesting a role in DNA repair .
  4. Alternative Splicing: YBX1 is involved in pre-mRNA alternative splicing regulation by binding to splice sites in pre-mRNA and regulating splice site selection .
  5. Extracellular Functions: The secreted form of YBX1 acts as an extracellular mitogen, stimulating cell migration and proliferation .
Role in Cancer

YBX1 is overexpressed in various human cancers and is recognized as an oncogenic gene associated with poor prognosis. Its functional diversity arises from its capacity to interact with a broad range of DNA and RNA molecules, implicating its involvement in diverse cellular processes. YBX1’s role in cancer includes promoting tumor progression, influencing the tumor microenvironment, and contributing to drug resistance .

Therapeutic Potential

Given its significant role in cancer, YBX1 is considered a potential target for cancer therapy. Ongoing clinical trials are exploring YBX1 as a target in breast cancer and lung cancer. The emerging role of YBX1 in neural input is also being investigated, with high levels of YBX1 associated with nerve cancer and neurodegenerative diseases .

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