XRCC3 Human
Escherichia Coli.
X-ray repair cross complementing protein 3, RAD51-like.
Greater than 85.0% as determined by SDS-PAGE.
Description
XRCC3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 366 amino acids (1-346 a.a.) and having a molecular mass of 40 kDa.
XRCC3 is fused to a 20 amino acid His tag at N-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Recombinant Human X-Ray Repair Cross Complementing Protein 3, also known as XRCC3, belongs to the RecA family and RAD51 subfamily. This protein plays a crucial role in homologous recombination, a process essential for maintaining chromosome stability and repairing DNA damage. XRCC3 effectively complements the Chinese hamster irs1SF cell line, a repair-deficient mutant exhibiting heightened sensitivity to various DNA-damaging agents and chromosomal instability.
Recombinant Human XRCC3, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 366 amino acids (specifically, amino acids 1 to 346). It possesses a molecular weight of 40 kDa. This XRCC3 variant is fused to a 20 amino acid His tag at its N-terminus and is purified using proprietary chromatographic techniques.
The XRCC3 solution is prepared at a concentration of 1 mg/ml and contains 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, and 0.4 M urea.
Repeated freezing and thawing of the solution should be avoided.
The purity of the XRCC3 protein is determined to be greater than 85.0% based on SDS-PAGE analysis.
X-ray repair cross complementing protein 3, RAD51-like.
Escherichia Coli.
MGSSHHHHHH SSGLVPRGSH MDLDLLDLNP RIIAAIKKAK LKSVKEVLHF SGPDLKRLTN LSSPEVWHLL RTASLHLRGS SILTALQLHQ QKERFPTQHQ RLSLGCPVLD ALLRGGLPLD GITELAGRSS AGKTQLALQL CLAVQFPRQH GGLEAGAVYI CTEDAFPHKR LQQLMAQQPR LRTDVPGELL QKLRFGSQIF IEHVADVDTL LECVNKKVPV LLSRGMARLV VIDSVAAPFR
CEFDSQASAP RARHLQSLGA TLRELSSAFQ SPVLCINQVT EAMEEQGAAH GPLGFWDERV SPALGITWAN QLLVRLLADR LREEEAALGC PARTLRVLSA PHLPPSSCSY TISAEGVRGT PGTQSH
Product Science Overview
XRCC3 is specifically involved in the homologous recombination repair (HRR) pathway of double-stranded DNA. This pathway is essential for repairing chromosomal fragmentation, translocations, and deletions. XRCC3 functions as part of the RAD51 paralog protein complex CX3, which acts in the BRCA1-BRCA2-dependent HR pathway . Upon DNA damage, the CX3 complex binds predominantly to the intersection of the four duplex arms of the Holliday junction (HJ) and to junctions of replication forks. This binding is crucial for the resolution of HJ and the processing of HR intermediates late in the DNA repair process .
Genomic instability is a hallmark of human tumors, and the dysfunction of the XRCC gene family, including XRCC3, is associated with the development of various cancers. Mutations in XRCC3 and its aberrant expression can result in abnormal DNA damage repair, contributing to the malignant progression of tumor cells . Research has shown that XRCC3 plays significant roles in diverse tumor types and is correlated with tumor therapeutic sensitivity .
Human recombinant XRCC3 is a form of the protein that is produced through recombinant DNA technology. This involves inserting the XRCC3 gene into a suitable expression system, such as bacteria or yeast, to produce the protein in large quantities. Recombinant XRCC3 is used in various research applications to study its function in DNA repair and its role in cancer development.
