XPNPEP1 Human
X-Prolyl Aminopeptidase-1 Human Recombinant
Cat. No.
BT24568
Source
Escherichia Coli.
Synonyms
X-Prolyl Aminopeptidase (Aminopeptidase P) 1, Soluble,
XPNPEPL, SAMP, X-Prolyl Aminopeptidase 1, Soluble, Aminoacylproline
Aminopeptidase, Cytosolic Aminopeptidase P, Soluble Aminopeptidase P, X-Pro
Aminopeptidase 1, EC 3.4.11.9, XPNPEPL1, X-Prolyl Aminopeptidase
(Aminopeptidase P)-Like, Aminopeptidase P, Cytosolic, Xaa-Pro Aminopeptidase 1,
XPNPEP, APP1, Xaa-Pro aminopeptidase 1.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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In Stock 
Description
XPNPEP1 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 655 amino acids (1-623 a.a) and
having a molecular mass of 73.4kDa.
XPNPEP1 is fused to a 32 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Product Specs
Introduction
X-Prolyl Aminopeptidase-1, also known as XPNPEP1, is a member of the peptidase M24B family. This enzyme, present in the cytosol, functions as a metalloaminopeptidase. Its primary role is to catalyze the removal of the N-terminal amino acid located next to a proline residue. Additionally, XPNPEP1 is involved in the degradation and maturation processes of tachykinins, neuropeptides, and peptide hormones.
Description
Recombinant human XPNPEP1, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 655 amino acids (specifically, amino acids 1 through 623). It possesses a molecular mass of 73.4 kDa. The protein is engineered with a 32-amino acid His-tag fused to its N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The XPNPEP1 protein is supplied in a solution at a concentration of 1 mg/ml. The solution contains phosphate-buffered saline (pH 7.4) and 20% glycerol.
Stability
For short-term storage (2-4 weeks), the product should be kept at 4°C. For longer storage, it is recommended to freeze the product at -20°C. To ensure long-term stability, adding a carrier protein (either 0.1% HSA or BSA) is advised. Repeated freezing and thawing should be avoided.
Purity
The purity of the XPNPEP1 protein is greater than 85.0%, as determined by SDS-PAGE analysis.
Synonyms
X-Prolyl Aminopeptidase (Aminopeptidase P) 1, Soluble,
XPNPEPL, SAMP, X-Prolyl Aminopeptidase 1, Soluble, Aminoacylproline
Aminopeptidase, Cytosolic Aminopeptidase P, Soluble Aminopeptidase P, X-Pro
Aminopeptidase 1, EC 3.4.11.9, XPNPEPL1, X-Prolyl Aminopeptidase
(Aminopeptidase P)-Like, Aminopeptidase P, Cytosolic, Xaa-Pro Aminopeptidase 1,
XPNPEP, APP1, Xaa-Pro aminopeptidase 1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH
SSGLVPRGSH MGSEFELRRQ ASMPPKVTSE LLRQLRQAMR NSEYVTEPIQ AYIIPSGDAH QSEYIAPCDC
RRAFVSGFDG SAGTAIITEE HAAMWTDGRY FLQAAKQMDS NWTLMKMGLK DTPTQEDWLV SVLPEGSRVG
VDPLIIPTDY WKKMAKVLRS AGHHLIPVKE NLVDKIWTDR PERPCKPLLT LGLDYTGISW KDKVADLRLK
MAERNVMWFV VTALDEIAWL FNLRGSDVEH NPVFFSYAII GLETIMLFID GDRIDAPSVK EHLLLDLGLE
AEYRIQVHPY KSILSELKAL CADLSPREKV WVSDKASYAV SETIPKDHRC CMPYTPICIA KAVKNSAESE
GMRRAHIKDA VALCELFNWL EKEVPKGGVT EISAADKAEE FRRQQADFVD LSFPTISSTG PNGAIIHYAP
VPETNRTLSL DEVYLIDSGA QYKDGTTDVT RTMHFGTPTA YEKECFTYVL KGHIAVSAAV FPTGTKGHLL
DSFARSALWD SGLDYLHGTG HGVGSFLNVH EGPCGISYKT FSDEPLEAGM IVTDEPGYYE DGAFGIRIEN
VVLVVPVKTK YNFNNRGSLT FEPLTLVPIQ TKMIDVDSLT DKECDWLNNY HLTCRDVIGK ELQKQGRQEA
LEWLIRETQP ISKQH.
Product Science Overview
Gene and Protein Structure
Biological Functions
XPNPEP1 is involved in several biological processes, including:
- Proteolysis: It contributes to the degradation of bradykinin, a peptide that causes blood vessels to dilate .
- Peptide Hormone Maturation: It aids in the maturation of peptide hormones and neuropeptides .
- Tachykinin Degradation: It plays a role in the degradation of tachykinins, which are neuropeptides involved in neurotransmission .
Clinical Significance
Mutations or deficiencies in the XPNPEP1 gene can lead to various disorders. For instance, a deficiency in XPNPEP1 results in the excretion of large amounts of imino-oligopeptides in urine . Additionally, diseases associated with XPNPEP1 include Deafness, Autosomal Recessive 84B and Spastic Paraplegia, Optic Atrophy, and Neuropathy .
Research and Applications
Recombinant human X-Prolyl Aminopeptidase-1 is used in research to study its role in peptide metabolism and its potential therapeutic applications. Understanding the enzyme’s function and regulation can provide insights into the treatment of related disorders and the development of therapeutic interventions.
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