XAF1 Human

XIAP Associated Factor 1 Human Recombinant
Cat. No.
BT983
Source
Escherichia Coli.
Synonyms
XIAP Associated Factor 1, BIRC4-Binding Protein, BIRC4BP, XIAPAF1, XIAP-Associated Factor 1, BIRC4 Binding Protein, HSXIAPAF1, XAF1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

XAF1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 161 amino acids (1-125) and having a molecular mass of 18.6 kDa.
XAF1 is fused to a 36 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
XIAP Associated Factor 1 (XAF1) is a protein that interacts with and counteracts the inhibitory effects of proteins belonging to the inhibitor of apoptosis (IAP) family. IAP proteins typically bind to and inhibit caspases, enzymes that are activated during apoptosis. The balance between IAPs and proteins like XAF1, which interfere with IAP activity, plays a crucial role in regulating the apoptotic signaling pathway. XAF1 specifically inhibits the anti-caspase activity of BIRC4, inducing its cleavage and inactivation independently of caspase activation. Moreover, XAF1 contributes to TNF-alpha-mediated apoptosis and is involved in apoptosis within trophoblast cells.
Description
Recombinant human XAF1, expressed in E. coli, is a single, non-glycosylated polypeptide chain composed of 161 amino acids (residues 1-125) with a molecular weight of 18.6 kDa. This protein includes a 36-amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The XAF1 solution is provided at a concentration of 1 mg/ml in a buffer containing 20 mM Tris-HCl (pH 8.0), 30% glycerol, 0.15 M NaCl, and 1 mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the product frozen at -20°C. To ensure optimal stability during long-term storage, adding a carrier protein such as HSA or BSA (0.1%) is advised. Avoid repeated freeze-thaw cycles.
Purity
The purity is determined to be greater than 90.0% using SDS-PAGE analysis.
Synonyms
XIAP Associated Factor 1, BIRC4-Binding Protein, BIRC4BP, XIAPAF1, XIAP-Associated Factor 1, BIRC4 Binding Protein, HSXIAPAF1, XAF1.
Source
Escherichia Coli.
Amino Acid Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMEGD FSVCRNCKRH VVSANFTLHE AYCLRFLVLC PECEEPVPKE TMEEHCKLEH QQVGCTMCQQ SMQKSSLEFH KANECQERPV ECKFCKLDMQ LSKLELHESY CGSRTELCQG CGQFIMHRML A.

Product Science Overview

Structure and Function

XAF1 is a zinc finger protein that binds to XIAP and neutralizes its function. XIAP is a member of the inhibitor of apoptosis (IAP) family, which inhibits apoptosis by directly inhibiting caspases, the enzymes responsible for the execution of apoptosis . By binding to XIAP, XAF1 promotes the activation of caspases, thereby facilitating apoptosis.

Role in Cancer

XAF1 functions as a tumor suppressor. It has been shown to cooperate with tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) to suppress the growth of colon cancer and trigger tumor regression . The combined restoration of XAF1 expression and TRAIL treatment has been found to significantly enhance apoptosis and inhibit tumor growth in colon cancer models .

Mechanism of Action

The mechanism by which XAF1 antagonizes XIAP involves the release of cytochrome c from mitochondria, which then activates caspases 3, 7, 8, and 9 . This leads to the down-regulation of XIAP, survivin, and c-IAP-2, which are proteins that inhibit apoptosis . The restoration of XAF1 expression directly induces apoptosis and enhances the apoptotic effects of TRAIL .

Clinical Implications

Given its role in promoting apoptosis and suppressing tumor growth, XAF1 is being investigated as a potential therapeutic target for cancer treatment. Strategies that involve the restoration of XAF1 expression or the use of recombinant XAF1 protein in combination with other apoptotic agents like TRAIL are being explored for their potential to treat various cancers .

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