WNT3A Antibody

Wnt-3a is a member of the Wnt family of proteins, which are highly conserved secreted signaling molecules that play crucial roles in various developmental processes, including cell fate determination, cell migration, cell polarity, neural patterning, and organogenesis. The Wnt signaling pathway is also implicated in the regulation of adult tissue homeostasis and has been associated with various diseases, including cancer.

Wnt-3a is a lipid-modified, secreted hydrophobic glycoprotein characterized by a conserved pattern of 24 cysteine residues . This protein is essential for cell signaling and is required for normal embryonic mesoderm development, formation of caudal somites, and normal morphogenesis of the developing neural tube . The protein’s structure allows it to interact with receptors of the Frizzled family and coreceptors of the low-density lipoprotein receptor-related protein family (LRP-5 or -6), or the Ryk atypical receptor tyrosine kinase .

Wnt-3a is known to induce alkaline phosphatase production in MC3T3-E1 mouse preosteoblast cells and Topflash reporter activity in HEK293T human embryonic kidney cells . These activities highlight its role in promoting osteoblast differentiation and its involvement in the canonical Wnt signaling pathway, which is crucial for various cellular processes.

Recombinant Wnt-3a proteins, both human and mouse, are widely used in research to study the Wnt signaling pathway and its implications in development and disease. These proteins are typically produced in Chinese Hamster Ovary (CHO) cell lines and are available in carrier-free formulations to avoid interference in experimental applications .

Mouse anti-human Wnt-3a antibodies are used in various research applications, including Western blotting, immunohistochemistry, and flow cytometry. These antibodies help in detecting and quantifying Wnt-3a protein levels in different biological samples, thereby aiding in the study of Wnt signaling and its role in various physiological and pathological processes .