WHSC1L1 Antibody
Histone-lysine N-methyltransferase NSD3, Mouse Anti Human
Cat. No.
BT27578
Source
Synonyms
Histone-lysine N-methyltransferase NSD3, Nuclear SET domain-containing protein 3,
Wolf-Hirschhorn syndrome candidate 1-like protein 1, Protein whistle, WHSC1-like 1 isoform 9 with methyltransferase activity to lysine, WHSC1-like protein 1, WHSC1L1, NSD3, DC28, pp14328, FLJ20353, MGC126766, MGC142029, DKFZp667H044.
Appearance
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Product Specs
Introduction
The WHSC1L1 gene, associated with the Wolf-Hirschhorn syndrome candidate-1 gene, encodes a protein containing PWWP (proline-tryptophan-tryptophan-proline) domains. While its exact function remains unclear, WHSC1L1 exhibits high expression levels in the brain, heart, and skeletal muscle, with lower levels observed in the liver and lungs.
Formulation
The antibody is supplied as a 1 mg/ml solution in a buffer consisting of PBS at pH 7.4 and 0.1% sodium azide.
Storage Procedures
For short-term storage (up to 1 month), store at 4°C. For extended periods, storage at -20°C is recommended. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The antibody remains stable for 12 months when stored at -20°C and for 1 month at 4°C.
Applications
The WHSC1L1 antibody has undergone rigorous testing through ELISA and Western blot analysis, ensuring its specificity and reactivity. However, optimization of the antibody concentration may be necessary for specific applications. For Western blot analysis, a dilution range of 1:1,000 to 1:3,000 is recommended, with an initial starting dilution of 1:2,000.
Synonyms
Histone-lysine N-methyltransferase NSD3, Nuclear SET domain-containing protein 3,
Wolf-Hirschhorn syndrome candidate 1-like protein 1, Protein whistle, WHSC1-like 1 isoform 9 with methyltransferase activity to lysine, WHSC1-like protein 1, WHSC1L1, NSD3, DC28, pp14328, FLJ20353, MGC126766, MGC142029, DKFZp667H044.
Purification Method
WHSC1L1 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
P2E9AT.
Immunogen
Anti-human WHSC1L1 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human WHSC1L1 amino acids 383-660 purified from E. coli.
Ig Subclass
Mouse IgG2b heavy chain and κ light chain.
Product Science Overview
Mouse Anti-Human Antibodies
Mouse anti-human antibodies are secondary antibodies that are generated by immunizing mice with human antigens. These antibodies are used in various immunological assays to detect human proteins. They are affinity-purified to ensure high specificity and are often conjugated with enzymes or fluorophores to facilitate detection .
Applications
Mouse anti-human antibodies are widely used in:
- Western Blotting: To detect specific proteins in a sample.
- ELISA: For quantifying the presence of antigens or antibodies in a sample.
- Flow Cytometry: To analyze the expression of cell surface and intracellular molecules.
- Immunohistochemistry: To visualize the distribution and localization of specific proteins within tissue sections .
Considerations
One important consideration when using mouse anti-human antibodies is the potential for the Human Anti-Mouse Antibody (HAMA) response. This is an immune reaction that can occur when human patients develop antibodies against the mouse antibodies, potentially leading to allergic reactions or interference in diagnostic assays .
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