WBP2 Human

WW Domain Binding Protein 2 Human Recombinant
Cat. No.
BT30343
Source
E.coli.
Synonyms
WW domain binding protein 2, WBP-2.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

WBP2 Human Recombinant produced in E. coli is a single polypeptide chain containing 284 amino acids (1-261) and having a molecular mass of 30.5 kDa.
WBP2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The protein known as WW domain-binding protein 2 (WBP2) functions as a coactivator for steroid hormone receptors. It possesses a single GRAM domain. The WW domain, characterized by its globular structure, consists of 38-40 semi-conserved amino acids found in proteins with diverse functions, including structural, regulatory, and signaling roles. This domain plays a crucial role in protein-protein interactions by binding to polyproline ligands. Notably, WBP2 interacts with the WW domain of Yes kinase-associated protein (YAP1) through its PY motifs.
Description
Recombinant WBP2, derived from humans and produced in E. coli, is a single polypeptide chain with a molecular weight of 30.5 kDa. It comprises 284 amino acids, spanning residues 1-261. The protein features a 23 amino acid His-tag fused to its N-terminus. Purification is achieved through proprietary chromatographic methods.
Physical Appearance
The solution is sterile, filtered, and colorless.
Formulation
The WBP2 solution is supplied at a concentration of 0.5 mg/ml. It is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.1 M NaCl, 1 mM DTT, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), the solution should be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. To maintain product integrity, avoid repeated freeze-thaw cycles.
Purity
SDS-PAGE analysis confirms a purity exceeding 90%.
Synonyms
WW domain binding protein 2, WBP-2.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMALNKNH SEGGGVIVNN TESILMSYDH VELTFNDMKN VPEAFKGTKK GTVYLTPYRV IFLSKGKDAM QSFMMPFYLM KDCEIKQPVF GANYIKGTVK AEAGGGWEGS ASYKLTFTAG GAIEFGQRML QVASQASRGE VPSGAYGYSY MPSGAYVYPP PVANGMYPCP PGYPYPPPPP EFYPGPPMMD GAMGYVQPPP PPYPGPMEPP VSGPDVPSTP AAEAKAAEAA ASAYYNPGNP HNVYMPTSQP PPPPYYPPED KKTQ

Product Science Overview

Structure and Function

The WW domain of WBP2 binds to the WW domain of Yes kinase-associated protein (YAP) through its PY motifs. This interaction is crucial for various cellular processes, including transcriptional regulation and signal transduction . WBP2 interacts with several WW-domain-containing proteins, such as:

  • Yes kinase-associated protein (YAP)
  • Paired box gene 8 (Pax8)
  • WW-domain-containing transcription regulator protein 1 (TAZ)
  • WW-domain-containing oxidoreductase (WWOX)

These interactions trigger downstream signaling pathways both in vitro and in vivo .

Role in Breast Cancer

WBP2 has been closely linked to the development of breast cancer. Studies have shown that the phosphorylated form of WBP2 can move into the nucleus and activate the transcription of estrogen receptor (ER) and progesterone receptor (PR), which are indicators of breast cancer development . Overexpression of WBP2 and activation of tyrosine phosphorylation upregulate signal cascades in the cross-regulation of the Wnt and ER signaling pathways in breast cancer .

When WBP2 binds with TAZ, it can accelerate migration and invasion, which are required for the transformed phenotypes of breast cancer cells. This suggests that WBP2 is a key player in regulating cell migration . Conversely, when WBP2 binds with WWOX, a tumor suppressor, ER transactivation and tumor growth can be suppressed .

Recombinant WBP2

Recombinant WBP2 is a form of the protein that is produced through recombinant DNA technology. This involves inserting the WBP2 gene into a suitable expression system, such as bacteria or yeast, to produce the protein in large quantities. Recombinant WBP2 is used in various research applications to study its structure, function, and role in diseases like breast cancer .

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