VZV ORF26

Varicella Zoster Virus ORF26 Recombinant
Cat. No.
BT10408
Source
Escherichia Coli.
Synonyms
Appearance
Purity
Varicella protein is >95% pure as determined by 10% PAGE (coomassie staining).
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

The E.Coli derived recombinant protein contains the VZV ORF26 immunodominant regions, amino acids 9-33, 184-208.

Product Specs

Introduction
Varicella-zoster virus (VZV) is closely related to the herpes simplex viruses (HSV), sharing significant genome homology. Both viruses possess similar envelope glycoproteins, including gB, gC, gE, gH, gI, gK, and gL. However, VZV lacks an equivalent to HSV gD. VZV virions exhibit a spherical shape with a diameter ranging from 150 to 200 nm. Their lipid envelope encapsulates a nucleocapsid composed of 162 capsomeres arranged in a hexagonal pattern. The viral DNA consists of a single, linear, double-stranded molecule with a length of 125,000 nucleotides. VZV is highly susceptible to disinfectants, particularly sodium hypochlorite. Effective treatments for VZV infection include antiviral drugs like acyclovir, vidarabine, and immunotherapies such as zoster immune globulin (ZIG).
Description
This recombinant protein is derived from E. coli and contains the immunodominant regions of VZV ORF26, specifically amino acids 9-33 and 184-208.
Purity
The purity of the Varicella protein exceeds 95%, as determined by 10% PAGE (coomassie staining).
Formulation
The Varicella protein is supplied in a buffer consisting of 25mM Tris-Hcl pH 7.2, 1mM EDTA, and 50% glycerol.
Stability
For optimal stability, the Varicella protein should be stored below -18°C. While it can remain stable at 4°C for up to one week, it is recommended to avoid repeated freeze-thaw cycles.
Applications
The Varicella Zoster antigen is well-suited for use in ELISA and Western blots. Its exceptional purity and specificity make it an excellent antigen for the detection of VZV with minimal cross-reactivity.
Source
Escherichia Coli.
Purification Method

Varicella was purified by proprietary chromatographic technique.

Specificity
Immunoreactive with sera of VZV-infected individuals.

Product Science Overview

Introduction to Varicella Zoster Virus (VZV)

Varicella Zoster Virus (VZV), also known as Human Herpesvirus 3 (HHV-3), is a member of the genus Varicellovirus in the Alphaherpesvirinae subfamily of the Herpesviridae family . VZV is the causative agent of chickenpox (varicella) in children and shingles (zoster) in adults. After the primary infection, the virus establishes latency in the sensory ganglia and can reactivate later in life to cause shingles .

Structure and Genome of VZV

The VZV genome is approximately 125 kilobases (kb) of linear double-stranded DNA containing around 71 open reading frames (ORFs) . The viral structure is similar to other alphaherpesviruses, consisting of two unique regions, unique long (UL) and unique short (US), each flanked by inverted repeats . The genome organization includes terminal repeat long (TRL) and internal repeat long (IRL) bordering the UL region, and terminal repeat short (TRS) and internal repeat short (IRS) bordering the US region .

ORF26 in VZV

ORF26 is one of the 71 ORFs in the VZV genome. It encodes a protein that is part of the viral tegument, which is a layer of proteins located between the viral envelope and the nucleocapsid . The ORF26 protein is known to be immunodominant, meaning it elicits a strong immune response in the host .

Recombinant VZV ORF26 Protein

Recombinant VZV ORF26 protein is produced by expressing the ORF26 gene in a bacterial system, such as Escherichia coli (E. coli), and purifying the protein using chromatographic techniques . The recombinant protein typically contains the immunodominant regions of the ORF26 protein, specifically amino acids 9-33 and 184-208 . This recombinant protein is used in various research and diagnostic applications, including the generation of antibodies that specifically target the ORF26 protein .

Applications of Recombinant VZV ORF26 Protein

The recombinant VZV ORF26 protein is valuable in several areas of research and diagnostics:

  • Immunization Protocols: It is used to generate antibodies that specifically target the ORF26 protein, which can be used in various immunological assays .
  • Diagnostic Tools: The recombinant protein can be used in diagnostic tests to detect the presence of VZV-specific antibodies in patient samples .
  • Vaccine Development: Research on the ORF26 protein can contribute to the development of vaccines against VZV .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

VZV gE
Varicella Zoster Virus gE Recombinant
Cat. No.
BT10331
Source
VZV ORF9
Varicella Zoster Virus ORF9 Recombinant
Cat. No.
BT10475
Source
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.