VTI1B Human

Vesicle Transport Through Interaction with t-SNAREs Homolog 1B Human Recombinant
Cat. No.
BT30075
Source
Escherichia Coli.
Synonyms
Vesicle transport through interaction with t-SNAREs homolog 1B, Vesicle transport v-SNARE protein Vti1-like 1, Vti1-rp1, VTI1B, VTI1, VTI1L, VTI1L1, VTI2, v-SNARE, VTI1-LIKE.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

VTI1B Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 231 amino acids (1-208 a.a) and having a molecular mass of 26.3kDa (Molecular weight on SDS-PAGE will appear higher).
VTI1B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
VTI1B, a v-SNARE protein, plays a crucial role in vesicle transport by interacting with t-SNAREs on target membranes. These interactions ensure specificity in vesicle trafficking and promote fusion between lipid bilayers. VTI1B is believed to be involved in the elevated secretion of cytokines associated with cellular senescence.
Description
Recombinant human VTI1B, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 231 amino acids (residues 1-208) with a molecular weight of 26.3 kDa. Note that the molecular weight observed on SDS-PAGE may be higher. The protein features a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
The VTI1B protein solution is provided at a concentration of 0.25 mg/ml in a buffer containing 20 mM Tris-HCl (pH 8.0), 30% glycerol, 0.15 M NaCl, and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), the protein can be stored at 4°C. For extended storage, freezing at -20°C is recommended. To further enhance stability during long-term storage, consider adding a carrier protein like 0.1% HSA or BSA. Repeated freezing and thawing cycles should be avoided.
Purity
The purity of the protein is greater than 85% as determined by SDS-PAGE analysis.
Synonyms
Vesicle transport through interaction with t-SNAREs homolog 1B, Vesicle transport v-SNARE protein Vti1-like 1, Vti1-rp1, VTI1B, VTI1, VTI1L, VTI1L1, VTI2, v-SNARE, VTI1-LIKE.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMASSAAS SEHFEKLHEI FRGLHEDLQG VPERLLGTAG TEEKKKLIRD FDEKQQEANE TLAEMEEELR YAPLSFRNPM MSKLRNYRKD LAKLHREVRS TPLTATPGGR GDMKYGIYAV ENEHMNRLQS QRAMLLQGTE SLNRATQSIE RSHRIATETD QIGSEIIEEL GEQRDQLERT KSRLVNTSEN LSKSRKILRS MSRKVTTNKL L.

Product Science Overview

Structure and Function

VTI1B is a v-SNARE (vesicle SNARE) protein that interacts with t-SNAREs (target SNAREs) on the target membrane. These interactions are critical for the specificity of vesicle trafficking and the promotion of lipid bilayer fusion . The protein contains a C-terminal transmembrane domain and two predicted coiled-coil regions . The human VTI1B protein is composed of 232 amino acids .

Biological Role

The primary function of VTI1B is to mediate vesicle transport pathways. This involves the transport of vesicles from the Golgi apparatus to the endosomes and lysosomes . VTI1B is also implicated in the secretion of cytokines associated with cellular senescence . The protein is expressed in all human tissues, indicating its fundamental role in cellular processes .

Genetic Information

The gene encoding VTI1B is located on chromosome 14q24.1 . It has several synonyms, including VTI1, VTI1L, VTI1L1, and VTI2 . The gene is highly conserved across species, with the human protein showing 29% identity to its yeast homolog .

Clinical Significance

Mutations or dysregulation of VTI1B can have significant implications for cellular function. For instance, disruptions in VTI1B interactions can lead to improper vesicle trafficking, which may contribute to various diseases . The protein’s role in cytokine secretion also suggests a potential link to inflammatory conditions and cellular aging .

Research and Applications

Research on VTI1B has provided insights into the molecular mechanisms of vesicle transport and membrane fusion. The protein’s interactions with clathrin-coated vesicles and its role in post-Golgi trafficking are areas of active investigation . Understanding these processes can inform the development of therapeutic strategies for diseases related to vesicle transport dysfunction.

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