VTA1 Antibody

Vps20-Associated 1 Homolog, Mouse Anti Human
Cat. No.
BT3243
Source
Synonyms
Vacuolar protein sorting-associated protein VTA1 homolog, Dopamine-responsive gene 1 protein, DRG-1, LYST-interacting protein 5, LIP5, SKD1-binding protein 1, SBP1, VTA1, C6orf55, DRG1My012, HSPC228.
Appearance
Sterile filtered colorless solution.
Purity
Usage
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Description

Product Specs

Introduction
Vps20-associated 1 homolog (VTA1), a member of the VTA1 family, participates in the endosomal multivesicular bodies (MVB) pathway. VTA1 interacts with several proteins, including CHMP1B, CHMP2A, CHMP5, VPS4B, KIAA0174 (IST1), and potentially CHMP3. It is considered a cofactor of VPS4A/B, which catalyzes membrane-associated ESCRT-III assemblies. Additionally, VTA1 plays a role in the sorting and down-regulation of EGFR and HIV-1 budding.
Physical Appearance
Sterile filtered colorless solution.
Formulation
1mg/ml in PBS, pH 7.4, with 10% Glycerol and 0.02% Sodium Azide.
Storage Procedures
Store at 4°C for up to 1 month. For longer storage periods, store at -20°C. Avoid freeze-thaw cycles.
Stability / Shelf Life
Stable for 12 months at -20°C and 1 month at 4°C.
Applications
This antibody has been validated for ELISA, Western blot analysis, Flow cytometry, and ICC/IF to ensure specificity and reactivity. However, optimal working concentrations may vary depending on the application and should be determined through titration.
Synonyms
Vacuolar protein sorting-associated protein VTA1 homolog, Dopamine-responsive gene 1 protein, DRG-1, LYST-interacting protein 5, LIP5, SKD1-binding protein 1, SBP1, VTA1, C6orf55, DRG1My012, HSPC228.
Purification Method
VTA1 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT14G10AT.
Immunogen
Anti-human VTA1 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human VTA1 protein 1-307 amino acids purified from E. coli.
Ig Subclass
Mouse IgG1 heavy chain and k light chain.

Product Science Overview

Structure and Function

VTA1 is a member of the VTA1 family and is known to interact with several other proteins involved in the MVB pathway, including CHMP1B, CHMP2A, CHMP5, VPS4B, KIAA0174 (IST1), and possibly CHMP3 . These interactions are vital for the proper functioning of the MVB pathway, ensuring that cellular waste and damaged proteins are efficiently processed and degraded.

Antibody Production

The Mouse Anti Human VTA1 antibody is a primary antibody produced in rabbits and is used in various research applications, including immunohistochemistry and Western blotting . This antibody is affinity isolated and is available in a buffered aqueous glycerol solution . It is part of the Prestige Antibodies® Powered by Atlas Antibodies product line, which is known for its extensive characterization and validation .

Applications

The Mouse Anti Human VTA1 antibody is used in several research techniques:

  • Immunoblotting: Used at concentrations ranging from 0.04-0.4 μg/mL .
  • Immunohistochemistry: Typically used at dilutions of 1:50-1:200 .

These applications are crucial for studying the expression and localization of VTA1 in various tissues and cells, providing insights into its role in cellular processes and disease mechanisms.

Research Significance

Understanding the function and interactions of VTA1 is essential for elucidating the mechanisms of the MVB pathway and its implications in cellular homeostasis and disease. Research involving VTA1 and its antibodies can contribute to the development of therapeutic strategies for diseases associated with dysfunctional protein degradation pathways.

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VTA1 Human
Vps20-Associated 1 Homolog Human Recombinant
Cat. No.
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Source
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