VPS4B Human
Vacuolar Protein Sorting 4 Homolog B Human Recombinant
Cat. No.
BT25013
Source
E.coli.
Synonyms
Vacuolar Protein Sorting 4 Homolog B (S. Cerevisiae), SKD1, Suppressor Of K+ Transport Defect 1, Cell Migration-Inducing Gene 1 Protein, Suppressor Of K(+) Transport Growth Defect 1,Vacuolar Protein Sorting 4B (Yeast), SKD1B, PS4-2, Cell Migration-Inducing 1, Vacuolar Protein Sorting 4B, Vacuolar Protein Sorting-Associated Protein 4B, EC 3.6.4.6, VPS42, Protein SKD1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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In Stock 
Description
VPS4B Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 468 amino acids (1-444 a.a) and having a molecular mass of 51.8kDa.
VPS4B is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
VPS4B is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Vacuolar protein sorting-associated protein 4B (VPS4B) is a member of the AAA (ATPases associated with diverse cellular activities) family. It plays a role in the final stages of the endosomal multivesicular bodies (MVB) pathway. VPS4B interacts with membrane-bound ESCRT-III complexes and facilitates their disassembly, likely in conjunction with membrane fission. Dominant negative mutations of VPS4B hinder vacuolar protein sorting and also interfere with HIV-1 and MLV budding.
Description
Recombinant human VPS4B, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 468 amino acids (with amino acids 1-444 present) and has a molecular weight of 51.8kDa. This VPS4B protein is fused to a 24 amino acid His-tag at its N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution, sterilized by filtration.
Formulation
The VPS4B protein is supplied in a solution at a concentration of 0.25mg/ml. The solution also contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 30% glycerol, and 1mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is advisable for long-term storage. Repeated freezing and thawing should be avoided.
Purity
Purity is determined to be greater than 90% using SDS-PAGE analysis.
Synonyms
Vacuolar Protein Sorting 4 Homolog B (S. Cerevisiae), SKD1, Suppressor Of K+ Transport Defect 1, Cell Migration-Inducing Gene 1 Protein, Suppressor Of K(+) Transport Growth Defect 1,Vacuolar Protein Sorting 4B (Yeast), SKD1B, PS4-2, Cell Migration-Inducing 1, Vacuolar Protein Sorting 4B, Vacuolar Protein Sorting-Associated Protein 4B, EC 3.6.4.6, VPS42, Protein SKD1.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMSSTSP NLQKAIDLAS KAAQEDKAGN YEEALQLYQH AVQYFLHVVK YEAQGDKAKQ SIRAKCTEYL DRAEKLKEYL KNKEKKAQKP VKEGQPSPAD EKGNDSDGEG ESDDPEKKKL QNQLQGAIVI ERPNVKWSDV AGLEGAKEAL KEAVILPIKF PHLFTGKRTP WRGILLFGPP GTGKSYLAKA VATEANNSTF FSISSSDLVS KWLGESEKLV KNLFQLAREN KPSIIFIDEI DSLCGSRSEN ESEAARRIKT EFLVQMQGVG VDNDGILVLG ATNIPWVLDS AIRRRFEKRI YIPLPEPHAR AAMFKLHLGT TQNSLTEADF RELGRKTDGY SGADISIIVR DALMQPVRKV QSATHFKKVR GPSRADPNHL VDDLLTPCSP GDPGAIEMTW MDVPGDKLLE PVVSMSDMLR SLSNTKPTVN EHDLLKLKKF TEDFGQEG.
Product Science Overview
Gene and Protein Structure
Function
VPS4B plays a crucial role in the late steps of the endosomal multivesicular bodies (MVB) pathway. It recognizes membrane-associated ESCRT-III assemblies and catalyzes their ATP-dependent disassembly, possibly in combination with membrane fission . This process is essential for the redistribution of ESCRT-III components to the cytoplasm for further rounds of MVB sorting .
Biological Processes
VPS4B is involved in various biological processes, including:
- Endosomal microautophagy
- Regulation of centrosome duplication
- Protein depolymerization
- Viral budding via host ESCRT complex
- Cholesterol transport
- Negative regulation of exosomal secretion
- Multivesicular body assembly
- Regulation of mitotic spindle assembly
- Cell division
- Endosomal transport
- Response to lipid
- Ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway
- Positive regulation of G2/M transition of mitotic cell cycle
- Positive regulation of exosomal secretion
- Septum digestion after cytokinesis
- Mitotic metaphase plate congression
- Endosome to lysosome transport via multivesicular body sorting pathway
- Cell cycle
- ESCRT III complex disassembly
- Macroautophagy
- Transport
- Positive regulation of viral process
- Vacuole organization
- Midbody abscission .
Clinical Significance
Research and Applications
Human recombinant VPS4B is used in various research applications to study its function and role in cellular processes. It is particularly valuable in understanding the mechanisms of intracellular protein trafficking and the endosomal sorting pathway.
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