VPS25 Human

Vacuolar Protein Sorting 25 Human Recombinant
Cat. No.
BT24622
Source
E.coli.
Synonyms
Vacuolar protein sorting 25 homolog (S. cerevisiae), vacuolar protein-sorting-associated protein 25, ELL-associated protein of 20 kDa, ESCRT-II complex subunit VPS25, Dermal papilla-derived protein 9, DERP9, EAP20, FAP20, MGC10540.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

VPS25 Human Recombinant produced in E. coli is a single polypeptide chain containing 200 amino acids (1-176) and having a molecular mass of 23.3kDa.
VPS25 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
VPS25 is a component of the ESCRT-II complex, which plays a crucial role in the endosomal sorting pathway. This pathway is responsible for transporting transmembrane proteins to the lysosome for degradation. VPS25, as part of ESCRT-II, facilitates the recruitment of the ESCRT-III complex, which is involved in the formation of multivesicular bodies (MVBs). These MVBs are essential for the proper sorting and degradation of cellular cargo.
Description
This product consists of a recombinant human VPS25 protein produced in E. coli. It is a single polypeptide chain with a molecular weight of 23.3 kDa, containing 200 amino acids, including a 24 amino acid His-tag at the N-terminus. The protein sequence spans from amino acid positions 1 to 176 of the human VPS25 protein. The recombinant protein has been purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The VPS25 protein is supplied at a concentration of 0.5 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 20% glycerol.
Stability
For short-term storage (up to 4 weeks), the product can be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is advisable for long-term storage. Repeated freezing and thawing should be avoided to maintain product integrity.
Purity
The purity of the VPS25 protein is greater than 85% as determined by SDS-PAGE analysis.
Synonyms
Vacuolar protein sorting 25 homolog (S. cerevisiae), vacuolar protein-sorting-associated protein 25, ELL-associated protein of 20 kDa, ESCRT-II complex subunit VPS25, Dermal papilla-derived protein 9, DERP9, EAP20, FAP20, MGC10540.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMAMSFE WPWQYRFPPF FTLQPNVDTR QKQLAAWCSL VLSFCRLHKQ SSMTVMEAQE SPLFNNVKLQ RKLPVESIQI VLEELRKKGN LEWLDKSKSS FLIMWRRPEE WGKLIYQWVS RSGQNNSVFT LYELTNGEDT EDEEFHGLDE ATLLRALQAL QQEHKAEIIT VSDGRGVKFF

Product Science Overview

Function and Mechanism

VPS25 is involved in the formation of multivesicular bodies (MVBs), which are specialized endosomes that contain vesicles. These MVBs are essential for the delivery of transmembrane proteins into the lumen of the lysosome for degradation . The ESCRT-II complex, which includes VPS25, is also likely involved in the recruitment of the ESCRT-III complex . This recruitment is crucial for the final stages of MVB formation and the sorting of endosomal cargo proteins into MVBs .

Additionally, the ESCRT-II complex may play a role in transcription regulation through its interaction with ELL, a transcription elongation factor . It is also suggested that the ESCRT-II complex could facilitate the budding of certain RNA viruses .

Recombinant VPS25

Recombinant VPS25 is a form of the protein that is produced through recombinant DNA technology. This involves inserting the VPS25 gene into a suitable expression system, such as Escherichia coli, to produce the protein in large quantities . The recombinant protein is typically purified to a high degree of purity, making it suitable for various biochemical and structural studies .

Applications and Research

Recombinant VPS25 is used in research to study its role in the ESCRT-II complex and its involvement in endosomal sorting and MVB formation. Understanding the function of VPS25 and the ESCRT-II complex can provide insights into cellular processes such as protein degradation, virus budding, and transcription regulation . This knowledge can also contribute to the development of therapeutic strategies for diseases associated with dysfunctional endosomal sorting, such as certain neurodegenerative diseases and cancers .

Associated Diseases

Mutations or dysregulation of the VPS25 gene have been linked to various diseases. For example, VPS25 is associated with Wolfram Syndrome 2, a rare genetic disorder characterized by diabetes insipidus, diabetes mellitus, optic atrophy, and deafness . Research into VPS25 and its associated pathways can help in understanding the molecular mechanisms underlying these diseases and potentially lead to the development of targeted therapies .

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