VOPP1 Human

Vesicular Overexpressed in Cancer, Prosurvival Protein 1 Human Recombinant
Cat. No.
BT29780
Source
Escherichia Coli.
Synonyms
Vesicular overexpressed in cancer prosurvival protein 1, EGFR-coamplified and overexpressed protein, ECop, Glioblastoma-amplified secreted protein, Putative NF-kappa-B-activating protein 055N, VOPP1, ECOP, GASP, FLJ20532, DKFZp564K0822.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

VOPP1 Human Recombinant fused with a 21 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 112 amino acids (82-172 a.a.) and having a molecular mass of 12kDa.
The VOPP1 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Vesicular overexpressed in cancer prosurvival protein 1 (VOPP1) is a protein that has been found to be overexpressed in certain types of cancer, including glioblastoma multiform and squamous cell carcinoma. VOPP1 plays a key role in regulating NF-kappaB signaling, a pathway involved in cell survival. The high levels of VOPP1 expression observed in tumors, particularly those with amplified EGFR, may contribute to resistance to apoptosis (cell death).
Description
Recombinant human VOPP1 is a protein produced in E. coli bacteria. It is a single, non-glycosylated polypeptide chain that contains 112 amino acids (specifically amino acids 82-172) and has a molecular mass of 12 kDa. This VOPP1 protein is fused with a 21 amino acid His tag at its N-terminus, which aids in purification. The protein is purified using proprietary chromatographic techniques.
Physical Appearance
Clear and colorless solution.
Formulation
The VOPP1 protein is supplied in a solution at a concentration of 0.5 mg/ml. The solution also contains 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 30% glycerol, and 0.2 M NaCl.
Stability
For short-term storage (2-4 weeks), the VOPP1 solution can be stored at 4°C. For longer-term storage, it is recommended to freeze the solution at -20°C. To further enhance stability during long-term storage, adding a carrier protein such as 0.1% HSA or BSA is advised. It is important to avoid repeated cycles of freezing and thawing the solution.
Purity
The purity of the VOPP1 protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
Vesicular overexpressed in cancer prosurvival protein 1, EGFR-coamplified and overexpressed protein, ECop, Glioblastoma-amplified secreted protein, Putative NF-kappa-B-activating protein 055N, VOPP1, ECOP, GASP, FLJ20532, DKFZp564K0822.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MRRRMYPPPL IEEPAFNVSY TRQPPNPGPG AQQPGPPYYT DPGGPGMNPV GNSMAMAFQV PPNSPQGSVA CPPPPAYCNT PPPPYEQVVK AK.

Product Science Overview

Discovery and Nomenclature

VOPP1 was initially identified in studies focusing on glioblastoma multiforme, a highly aggressive brain tumor. The protein was named GASP due to the presence of a signal sequence in its amino acid structure, suggesting its entry into the classical secretory pathway . The term ECOP was derived from its co-amplification with the Epidermal Growth Factor Receptor (EGFR) gene, which is located at the 7p11.2 locus, a region frequently amplified in glioblastomas .

Expression and Localization

VOPP1 is highly expressed in several types of human cancers, including squamous cell carcinoma, gastric cancer, and glioblastoma . It is also found in breast carcinoma, pancreatic carcinoma, and lymphoma . The protein is localized intracellularly, specifically in cytoplasmic vesicles. It does not co-localize with mitochondria or peroxisomes but shows partial co-localization with perinuclear lysosomes, endocytosis, and autophagy markers .

Functional Role in Cancer

VOPP1 plays a crucial role in promoting cell survival and proliferation. In hepatocellular carcinoma (HCC), VOPP1 expression is significantly higher in cancerous tissues compared to adjacent non-cancerous tissues . Silencing VOPP1 using shRNA has been shown to inhibit cell proliferation and tumor growth while inducing apoptosis both in vitro and in vivo . This suggests that VOPP1 may contribute to cancer progression by targeting the MAPK and mTOR signaling pathways .

Potential as a Therapeutic Target

Given its role in cancer cell survival and proliferation, VOPP1 is considered a potential molecular target for cancer therapy. By understanding the mechanisms through which VOPP1 influences cancer progression, researchers hope to develop targeted therapies that can inhibit its function and thereby reduce tumor growth and improve patient outcomes .

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