VNN1 Human
Description
Product Specs
Vanin 1 (VNN1) is a protein belonging to the vanin family, known for its similarity to biotinidase. This family includes proteins that are either secreted or membrane-bound, some of which are involved in hematopoietic cell movement. Although VNN1 doesn't have proven biotinidase activity, it exhibits pantetheinase activity, potentially playing a role in oxidative stress response. VNN1, similar to its counterpart in mice, is likely a GPI-anchored cell surface molecule. In mice, VNN1 is expressed by cells surrounding the thymus and influences the movement of T-cell precursors to the thymus. VNN1 functions as an amidohydrolase, specifically breaking down a carboamide bond in D-pantetheine, leading to the recycling of pantothenic acid (vitamin B5).
To prepare a working stock solution, it is advisable to add deionized water to the lyophilized pellet, aiming for a concentration of approximately 0.5mg/ml. Allow sufficient time for the pellet to dissolve completely. It's important to note that VNN1 is not sterile. Prior to using it in cell culture, filter the product using a sterile filter of appropriate pore size.
Product Science Overview
Vanin 1, also known as VNN1, is a cell membrane protein that belongs to the CN hydrolase family and the BTD/VNN subfamily. It is also referred to as Pantetheinase, Pantetheine hydrolase, and Vascular non-inflammatory molecule 1 . This protein plays a crucial role in various biological processes, including the regulation of stress responses and the metabolism of pantothenic acid (vitamin B5).
Vanin 1 contains one CN hydrolase domain and is widely expressed in various tissues, with higher expression levels observed in the spleen, kidney, and blood . It is also overexpressed in lesional psoriatic skin . The recombinant human Vanin 1 protein is typically produced in HEK293 cells and consists of 480 amino acids with a predicted molecular mass of 53.7 kDa . Due to glycosylation, it migrates as an approximately 70-75 kDa band in SDS-PAGE under reducing conditions .
Vanin 1 functions as a pantetheinase, catalyzing the hydrolysis of pantetheine to pantothenic acid (vitamin B5) and cysteamine . This enzymatic activity is essential for the recycling of pantothenic acid and the release of cysteamine, which plays a role in various metabolic processes . Vanin 1 is also involved in the regulation of late adhesion steps of thymus homing under physiological, non-inflammatory conditions .
The absence of Vanin 1 limits the production of proinflammatory signals by intestinal epithelial cells, highlighting its role in regulating the response to stress . Additionally, Vanin 1 is a member of the Vanin family of proteins, which share extensive sequence similarity with each other and with biotinidase . Some members of this family have been reported to participate in hematopoietic cell trafficking .
Recombinant human Vanin 1 protein is produced using DNA sequences encoding the human VNN1 without the propeptide, fused with a polyhistidine tag at the C-terminus . The protein is typically lyophilized from sterile PBS and can be reconstituted for various research applications . It is used in studies related to its enzymatic activity, tissue distribution, and role in disease processes.
