Introduction
VEGF-C152S is a mutated variant of VEGF-C where the second conserved cysteine residue is substituted with a serine residue. This alteration renders VEGF-C152S specifically active towards VEGFR-3/FLT-4 while abolishing its binding and activation of VEGFR-2/KDR, unlike the wild-type VEGF-C. Consequently, VEGF-C152S does not induce vascular permeability or stimulate capillary endothelial cell migration, both of which are VEGF-like effects mediated through VEGFR-2. VEGF-C, also recognized as Vascular Endothelial Growth Factor Related Protein (VRP), belongs to the VEGF growth factor family and shares significant homology with VEGF-D. The rat VEGF-C cDNA encodes a precursor protein of 416 amino acids, exhibiting near-identicality to the mouse VEGF-C protein. Similar to VEGF-D, VEGF-C possesses a VEGF homology domain located within the central third of the precursor molecule, flanked by extended N- and C-terminal regions. In adult tissues, VEGF-C exhibits high expression levels in the heart, placenta, ovary, and small intestine. The recombinant rat VEGF-C, devoid of the N- and C-terminal extensions and encompassing only the central VEGF homology domain, primarily exists as non-covalently linked dimers. This protein interacts with both VEGFR-2/KDR and VEGFR-3/FLT-4 receptors. Given the strong expression of VEGFR-3 in lymphatic endothelial cells, it is hypothesized that VEGF-C plays a role in regulating the growth and/or differentiation of lymphatic endothelium. Although recombinant rat VEGF-C can induce mitogenic effects on vascular endothelial cells, its potency is significantly lower compared to VEGF-A.
Description
Recombinant Rat Vascular Endothelial Growth Factor -C 152 is a 152 amino acid protein with a C-terminal His-tag (6x His) added through fusion. Due to glycosylation, it appears as an 18-24 kDa protein under reducing conditions in SDS-PAGE.
Physical Appearance
White lyophilized powder, sterile and filtered.
Formulation
The protein solution, at a concentration of 1mg/ml in BSA, was lyophilized.
Solubility
Reconstitute the lyophilized Vascular Endothelial Growth Factor C 152 with sterile 18MΩ-cm H2O to a concentration of at least 100µg/ml. This solution can be further diluted with other aqueous solutions.
Stability
Lyophilized Vascular Endothelial Growth Factor-C152 is stable at room temperature for 3 weeks but should be stored desiccated below -18°C. Once reconstituted, store VEGF-C 152 at 4°C for 2-7 days. For long-term storage, keep it below -18°C. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeding 90.0% as determined by: (a) RP-HPLC analysis. (b) SDS-PAGE analysis.
Biological Activity
The biological activity is assessed by measuring its ability to stimulate VEGFR-3/FLT-4 receptor phosphorylation in porcine aortic endothelial cells (PAE/FLT-4 cells). Typically, the ED50 for this effect falls within the range of 150-300 ng/ml, corresponding to a specific activity of 3.3-6.6KUnits/mg.
Synonyms
VEGF-C152, Vascular endothelial growth factor C 152, VRP, Flt4 ligand, Flt4-L.
Source
Sf9, Insect Cells.
