VBP1 Human
Description
VBP1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Von Hippel-Lindau Binding Protein 1 (VBP1) is a crucial protein that interacts with the Von Hippel-Lindau (VHL) tumor suppressor protein. This interaction is significant in the regulation of various cellular processes, including transcriptional elongation and protein transport. The recombinant form of VBP1, produced through genetic engineering techniques, is widely used in research to study its functions and interactions.
VBP1 belongs to the prefoldin subunit alpha family and functions as a chaperone protein. It assists in the proper folding and stabilization of other proteins, particularly the VHL protein. VBP1 is believed to play a role in the transport of the VHL protein from the perinuclear granules to the nucleus or cytoplasm .
The VHL protein is involved in the down-regulation of transcriptional elongation by preventing the association of elongin B and C with the catalytic subunit elongin A. Mutations in the VHL gene lead to Von Hippel-Lindau disease, characterized by various neoplasias, including hemangioblastomas, renal cell carcinomas, and pheochromocytomas . VBP1 binds to the VHL protein, forming an intracellular complex that is essential for the proper functioning of the VHL protein .
Studies have shown that VBP1 is ubiquitously expressed in various tissues. In fetal stages, it is primarily expressed in the central nervous system, retina, and liver. In adult tissues, VBP1 expression is widespread, with specific cell types in the brain, eye, kidney, and intestine showing higher levels of expression . The gene encoding VBP1 is located in a region homologous to human Xq28 .
Recombinant human VBP1 is produced using E. coli expression systems. The recombinant protein typically includes an N-terminal His-tag to facilitate purification. It is used in various research applications to study the protein’s structure, function, and interactions. The recombinant VBP1 protein is purified using conventional chromatography techniques and is available in high purity for research purposes .
Recombinant VBP1 is a valuable tool in molecular biology and biomedical research. It is used to investigate the mechanisms of VHL-related diseases, study protein-protein interactions, and explore the role of chaperone proteins in cellular processes. Understanding the function and regulation of VBP1 can provide insights into the development of therapeutic strategies for diseases associated with VHL mutations .
