VAPA Human
VAMP Associated Protein A 33kDa Human Recombinant
Cat. No.
BT6051
Source
Escherichia Coli.
Synonyms
hVAP-33, VAP-33, VAP-A, VAP33, Vesicle-associated membrane protein-associated protein A, VAMP-associated protein A, VAMP-A, 33 kDa VAMP-associated protein, VAPA.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
VAPA Human Recombinant produced in E.Coli is a single, non-glycosylated,
polypeptide chain containing 264 amino acids (1-227 a.a.) and having a molecular mass of 29.8 kDa. VAPA is fused to 37 amino acid His Tag and purified by proprietary chromatographic techniques.
Product Specs
Introduction
VAPA, a type IV membrane protein, plays a crucial role in vesicle trafficking. It resides in the plasma membrane and intracellular vesicles, exhibiting connections with the cytoskeleton. This protein participates in various cellular processes, including membrane fusion, protein complex assembly, and cell motility. Notably, VAPA is essential for regulating the subcellular localization of protrudin and its influence on neurite outgrowth.
Description
Recombinant Human VAPA, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 264 amino acids (1-227 a.a.). With a molecular mass of 29.8 kDa, it is fused to a 37 amino acid His Tag and purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized through filtration.
Formulation
The VAPA solution is prepared with 20mM Tris buffer at pH 8, 1mM DTT (dithiothreitol), and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the solution should be kept at 4°C. For longer periods, it's recommended to store it frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freezing and thawing should be avoided.
Purity
SDS-PAGE analysis confirms a purity exceeding 85%.
Synonyms
hVAP-33, VAP-33, VAP-A, VAP33, Vesicle-associated membrane protein-associated protein A, VAMP-associated protein A, VAMP-A, 33 kDa VAMP-associated protein, VAPA.
Source
Escherichia Coli.
Amino Acid Sequence
RGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMAS ASGAMAKHEQ ILVLDPPTDL KFKGPFTDVV TTNLKLRNPS DRKVCFKVKT TAPRRYCVRP NSGIIDPGST VTVSVMLQPF DYDPNEKSKH KFMVQTIFAP PNTSDMEAVW KEAKPDELMD SKLRCVFEMP NENDKLNDME PSKAVPLNAS KQDGPMPKPH SVSLNDTETR KLMEECKRLQ GEMMKLSEEN RHLRDEGLRL RKVAHSDKPG STSTASFRDN VTSP.
Product Science Overview
Structure and Function
Biological Roles
VAPA plays several crucial roles in cellular processes:
- Membrane Trafficking: VAPA is involved in vesicle trafficking and membrane fusion. It interacts with SNARE proteins, which are essential for the fusion of vesicles with target membranes .
- Lipid Transport and Metabolism: VAPA regulates lipid transport and metabolism by interacting with proteins containing the FFAT motif, such as STARD3 and OSBPL3. This interaction facilitates cholesterol transfer from the ER to endosomes .
- Unfolded Protein Response (UPR): VAPA is implicated in the UPR, a cellular stress response related to the ER. This response is activated in conditions of ER stress to restore normal function by halting protein translation and activating signaling pathways that lead to increased production of molecular chaperones involved in protein folding .
Clinical Significance
Mutations or dysregulation of the VAPA gene have been associated with various diseases, including:
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