VAMP2 Mouse
Vesicle-associated membrane protein 2, VAMP-2, Synaptobrevin-2, Vamp2, Syb2.
Greater than 90.0% as determined by SDS-PAGE.
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Description
VAMP2 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 118 amino acids (1-94 a.a) and having a molecular mass of 12.8kDa. VAMP2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Vesicle-associated membrane protein 2, VAMP-2, Synaptobrevin-2, Vamp2, Syb2.
MGSSHHHHHH SSGLVPRGSH MGSHMSATAA TVPPAAPAGE GGPPAPPPNL TSNRRLQQTQ AQVDEVVDIM RVNVDKVLER DQKLSELDDR ADALQAGASQ FETSAAKLKR KYWWKNLK.
Product Science Overview
Synaptobrevin-2, also known as Vesicle-associated membrane protein 2 (VAMP2), is an integral membrane protein that plays a crucial role in the process of synaptic vesicle fusion. This protein is predominantly expressed in the brain and is essential for the proper functioning of synaptic transmission.
Synaptobrevin-2 is an 18 kDa protein localized to the cytoplasmic surface of synaptic vesicles. It consists of a proline-rich N-terminal region, a highly conserved hydrophilic domain, followed by a transmembrane anchor and a C-terminal . The N-terminal domain of Synaptobrevin-2 forms a specific SNARE complex with the target membrane-associated t- or Q-SNAREs syntaxin 1 and SNAP-25 . This complex is essential for the fusion of synaptic vesicles with the plasma membrane, a process that is critical for neurotransmitter release.
Recombinant Synaptobrevin-2 from mouse is produced in Escherichia coli (E. coli) as a single, non-glycosylated polypeptide chain containing 118 amino acids . The recombinant protein is fused to a 24 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques . The molecular mass of the recombinant protein is approximately 12.8 kDa .
Recombinant Synaptobrevin-2 is used extensively in laboratory research to study the mechanisms of synaptic vesicle fusion and neurotransmitter release. It is also employed in various biochemical assays to investigate the interactions between SNARE proteins and other components of the synaptic vesicle fusion machinery .
The recombinant Synaptobrevin-2 protein is typically supplied as a sterile filtered clear solution in phosphate-buffered saline (PBS) with 1 mM EDTA, 0.1 mM PMSF, and 10% glycerol . For short-term storage, it can be kept at 4°C for 2-4 weeks. For long-term storage, it is recommended to store the protein at -20°C with the addition of a carrier protein such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA) to prevent multiple freeze-thaw cycles .
Studies have shown that deletion of synaptobrevin/vesicle-associated membrane protein significantly decreases, but does not abolish, spontaneous and evoked synaptic vesicle exocytosis . The closely related R-SNARE protein cellubrevin can rescue synaptic transmission in synaptobrevin-deficient neurons . Additionally, structural studies have revealed that the SNARE motif of Synaptobrevin-2 must be tightly coupled to the transmembrane region to mediate Ca²⁺-evoked exocytosis effectively .
