USP15 Human

The USP15 gene encodes a protein that is involved in various cellular processes, including the regulation of the transforming growth factor-beta (TGF-β) signaling pathway. The protein is known to associate with the COP9 signalosome, a multi-protein complex involved in various cellular and developmental processes .

USP15 functions as a hydrolase that removes conjugated ubiquitin from target proteins, thereby regulating several pathways such as the TGF-β receptor signaling, NF-kappa-B, and RNF41/NRDP1-PRKN pathways . It acts as a key regulator of the TGF-β receptor signaling pathway by promoting the deubiquitination of monoubiquitinated receptor-activated SMAD transcription factors (SMAD1, SMAD2, and/or SMAD3), which alleviates the inhibition of R-SMADs and promotes the activation of TGF-β target genes .

Recombinant human USP15 is typically expressed in baculovirus-infected Sf9 insect cells using an N-terminal GST tag. The recombinant protein is supplied in a sterile buffer and is highly purified for use in various research applications . The protein has a molecular mass of approximately 130 kDa and is formulated to maintain stability and activity during storage and handling .

Recombinant USP15 is used in various biochemical assays to study its enzymatic activity and regulatory functions. It is also utilized in research focused on understanding the mechanisms of deubiquitination and its impact on cellular signaling pathways. The protein’s role in TGF-β signaling makes it a valuable tool for investigating the molecular basis of diseases associated with dysregulated TGF-β signaling, such as cancer and fibrosis .

For optimal storage, recombinant USP15 should be aliquoted into smaller quantities after centrifugation and stored at -70°C. It is important to avoid repeated freeze/thaw cycles to maintain the protein’s activity .